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A Rapid Method for the Purification of RNA Polymerase Holoenzyme From Escherichia Coli

A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gell filtration chromatography gave 95% pure holoenzyme. The enzyme kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures.

Identiferoai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-14051
Date01 January 1990
CreatorsMehrpouyan, Majid, Champney, W. Scott
PublisherDigital Commons @ East Tennessee State University
Source SetsEast Tennessee State University
Detected LanguageEnglish
Typetext
SourceETSU Faculty Works

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