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Global RNA profiling of susceptible and tolerant genotypes of Brassica napus infected with Sclerotinia sclerotiorum and prediction and functional characterization of novel regulators of plant defense

Brassica napus (L.) contributes over $19 billion dollars each year to the Canadian economy. However, yields are constantly threatened by Sclerotinia sclerotiorum (Lib) de Bary, the fungus responsible for Sclerotinia stem rot. To date, there are no global RNA profiling data or gene regulatory analyses of plant tissues directly at the main site of foliar infection in the B. napus-S. sclerotiorum pathosystem. Using RNA sequencing and a gene regulatory analysis, I discovered putative transcriptional regulators of biological processes associated with the tolerant phenotype of B. napus cv. Zhougyou821 including subcellular localization of proteins, pathogen detection, and redox homeostasis. Functional characterization of Arabidopsis mutants identified a number of genes that contribute directly to plant defense to S. sclerotiorum. Together this research amounts to the expansion of our understanding of the B. napus-S. sclerotiorum pathosystem and a valuable resource to help protect B. napus crops from virulent pathogens such as S. sclerotiorum. / October 2016

Identiferoai:union.ndltd.org:MANITOBA/oai:mspace.lib.umanitoba.ca:1993/31862
Date January 2016
CreatorsGirard, Ian
ContributorsBelmonte, Mark (Biological Sciences) Fernando, Dilantha (Plant Sciences), Belmonte, Mark (Biological Sciences) Fernando, Dilantha (Plant Sciences) Chen, Guanqun (Biological Sciences)
PublisherFrontiers, Molecular Plant
Source SetsUniversity of Manitoba Canada
Detected LanguageEnglish

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