Return to search

Cytopathology and Release of an RNA Virus From a Strain of Trichomonas Vaginalis

A strain of Trichomonas vaginalis infected with a double-stranded RNA virus showed pronounced cytopathology in the form of giant syncytia generated by the recruitment of single cells. The giant cells ultimately lysed, releasing virus into the culture medium. In the infected cells, clusters of electron-dense particles resembling viral structures were found in the cytoplasm. In addition, distinctive inclusions composed of similar particles were present in the nuclei of some cells. Double-stranded viral RNA of 5.5 kbp was demonstrated in both cytoplasmic and nuclear fractions from these cells. Viral particles collected from the cell-free culture supernatant were of the same shape and size as the RNA virus isolated from a strain of T. vaginalis described previously (Wang and Wang, Journal of Biological Chemistry, 260: 3697-3702, 1985; Wang and Wang, Proceedings of the National Academy of Sciences of the U.S.A. 83: 7956-7986) which does not show this cytopathology.

Identiferoai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-13074
Date01 January 1995
CreatorsChampney, W. Scott, Curtis, Sherill K., Samuels, Robert
PublisherDigital Commons @ East Tennessee State University
Source SetsEast Tennessee State University
Detected LanguageEnglish
Typetext
SourceETSU Faculty Works

Page generated in 0.0017 seconds