Silene latifolia and Silene dioica are closely related dioecious plant species where sex is determined by the presence of sex chromosomes, X and Y. The sex chromosomes of S. latifolia and S. dioica are younger (10-20 million years) than in mammals (about 200 million years). These two species are thus important models for the study of early stages of sex chromosomes evolution. In this work a group of genes linked to sex chromosomes (Sl3, Sl7, DD44) was analyzed. These genes were de-novo isolated from S. dioica by screening of the BAC library. The expression of these genes in different tissues was compared using Real-Time PCR and HRM in S. latifolia and S. dioica. Using the low-copy FISH (fluorescence in situ hybridization) method, a physical map for individual genes was created. This map is the most detailed physical map created for closely related Silene species.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:430214 |
| Date | January 2017 |
| Creators | Machálková, Zuzana |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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