Ancient DNA analysis of archaeological wheat remains may serve to clarify
unknown or controversial points in the history of wheat. In the first part of this study,
extraction and amplification of DNA from Anatolian charred ancient wheat seeds obtained
from different locations and ages was attempted. None of the our extraction samples yielded
any PCR amplification. The possible reasons for this result were investigated by constructing
an artificial charring experiment. The results suggest that the chances of obtaining DNA
from the charred archaeological samples used in this study by the methods used are very
low. Moreover, strong PCR inhibition by these charred seed extracts was observed.
The second part of the study aimed to develop new DNA based markers for ancient
wheat DNA analysis. Markers linked to the brittle rachis character exhibiting domestication
status were sought, but no result was obtained. Primers targeting plasmon sequences were
developed and tested. A primer pair amplifying a 400 bp portion of the chloroplast TrnLTrnF
intergenic region was focused upon. A short piece of this region was amplified using
ancient wheat DNA extracted in another study. This short piece appeared non-polymorphicupon sequencing. The sequence spanning a wider portion of this region contained a number
of length polymorphisms. Phylogenetic reconstruction using maximum parsimony showed
that these polymorphisms were able to distinguish wheat taxa at the maternal ancestor level.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/1082957/index.pdf |
| Date | 01 January 2003 |
| Creators | Somel, Mehmet |
| Contributors | Kence, Aykut |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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