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A Quantitative Radioimmunoassay for Phosphoglucose Isomerase and Its Utilization in Detecting Cross-Reactive Material in Variant Forms of Phosphoglucose Isomerase and in Human Tissues

A method for purification and radiolabelling phosphoglucose isomerase was devised in order to develop a sensitive quantitative radioimmunoassay for the detection of the enzyme irrespective of its catalytic activity. For four genetic variants of PGI no difference in the molecular specific activity was observed. In one variant (PGI-Denton), liver and heart tissue extracts, and in mature erythrocytes (as compared to normal erythrocytes), a decreased molecular specific activity was observed which initially may imply that these samples contain cross-reactive material which is not catalytically active.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc663113
Date05 1900
CreatorsPurdy, Kimberly L.
ContributorsGracy, Robert W., Hatten, Betty A.
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvii, 69 leaves : ill., Text
RightsPublic, Purdy, Kimberly L., Copyright, Copyright is held by the author, unless otherwise noted. All rights

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