Three studies were conducted to determine the effects of
cyclopropenoid fatty acids (CPFA) on the membrane components of livers
of rainbow trout (Salmo gairdneri). In the first study, ¹⁴C-sterculic
acid was administered by intraperitoneal injection into rainbow trout
and the trout maintained for 72 hours. The labelled sterculic acid
was found in choline phospholipids (CP) and ethanolamine phospholipids
(EP). Smaller amounts of label were found in other microsomal
membrane lipid components. No label was found associated with the
proteins of the microsomal membrane. Phospholipase A₂ treatment of
isolated CP and EP showed ¹⁴C-sterculic acid to be preferentially
esterified to the 1-position of the glycerol backbone.
In the second study, the cleavable bifunctional protein
crosslinking reagent dimethyl 3,3'-dithiobispropionimidate-2HCl (DTBP)
was used in an attempt to study alterations in the spatial arrangement
of proteins in liver microsomal and plasma membranes that might be
induced by dietary CPFA. The use of this reagent failed to yield a
clear picture of protein-protein interactions in the microsomal
membrane due to the formation of high molecular weight aggregates that
were not resolvable on polyacrylamide gels. On the other hand, the
use of DTBP failed to crosslink the proteins of the plasma membrane.
In the third study, two-dimensional polyacrylamide gel
electrophoresis was used to assess the effects of dietary CPFA on
protein composition of trout liver microsomal and plasma membranes.
Proteins were separated in the first dimension on the basis of their
isoelectric points and in the second dimension on the basis of their
molecular weights. No major alterations in the composition of liver
microsomal or plasma membranes were found to be induced by dietary
CPFA. / Graduation date: 1983
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/25962 |
| Date | 20 September 1982 |
| Creators | Einerson, Mark A. |
| Contributors | Selivonchick, Daniel P. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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