Raspberry bushy dwarf virus (RBDV) is the sole member of the idaeovirus genus
of plant viruses. It is a pollen-borne virus that economically impacts both red and black
raspberries worldwide. Three strains of the bipartite RBDV have been reported. The
common strain found in North America, South America, Australia, New Zealand and
Europe and the resistance breaking strain found only in Europe are serologically
indistinguishable while the black raspberry strain from North America is distinct.
Resistance to RBDV is conferred by a single dominant gene, Bu. The resistance
breaking strain is able to infect all raspberry cultivars containing the Bu loci. The
sequence of a full-length clone of the common strain was determined and compared to
the previously published sequence of the resistance breaking strain. Nucleotide
homology between the two isolates was 97.6% for RNA 1 and 97.6% for RNA 2.
Comparison of the predicted RNA 1 protein product of the two strains showed 97.9%
homology. The predicted amino acid sequence of the movement protein and the coat
protein from RNA 2 demonstrated 98.6% and 98.5% identity respectively.
The differential rate of virus spread under field conditions in the Pacific
Northwest was also investigated. The possibility of higher temperatures inhibiting virus infected
pollen was explored by using in vitro germination and enzymatic staining
procedures. Results from a two year study of virus-infected and virus-free raspberry
pollen viability from both the Oregon and southern Washington region and the northern
Washington and southern British Columbia region suggest temperature does not affect
pollen germination or viability.
Further studies were conducted to determine if a virus-degrading agent could be
present on bee-stored raspberry pollen that becomes active only at higher temperatures.
RBDV infected pollen from hives in northern Washington was collected and tested by
ELISA after receiving either no heating or 32 hours of heating at 20, 30 or 40 C.
Preliminary results indicate no such agent exists in red raspberry pollen from hives in the
Pacific Northwest. / Graduation date: 2000
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/33212 |
| Date | 08 July 1999 |
| Creators | Taylor, Susannah M. |
| Contributors | Martin, Robert R. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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