The role of the dorsal hippocampus, entorhinal cortex, amygdala and fimbria fornix in spatial discrimination was investigated using temporary inactivation and lesioning methods. Spatial learning was tested in a conditioned cue preference (CCP) paradigm involving three phases of behavioral testing on an 8-arm radial maze. In the first phase (pre-exposure) rats were given unreinforced pre-exposure trials in which they were free to move on two adjacent arms of the maze on three consecutive days. Rats were then alternately confined to the ends of the arms for eight days (training), one arm that contained food (Paired-arm) and one that did not (Unpaired-arm). The rats were then given a choice between the two arms with no food present. Only when given unreinforced pre-exposure trials did rats spend more time in the Paired-arm than in the Unpaired-arm, a CCP. Rats with muscimol induced inactivation of the dorsal hippocampus during unreinforced pre-exposure acquired a CCP for the Paired-arm but were impaired with hippocampal inactivation during training or testing. Inactivation of the entorhinal cortex resulted in impairment in all phases of the paradigm. Inactivation of the dorsal hippocampus in the Unpaired but not Paired-arm only resulted in impairment. The effects of dorsal hippocampus inactivation in either the Paired or Unpaired arms were reversed in rats with combined amygdala lesions\dorsal hippocampus inactivation. Rats with fimbria fornix\entorhinal cortex disconnections during pre-exposure were also impaired. These results reveal that spatial learning may rely on the interactions between the hippocampus, entorhinal cortex, amygdala, and fimbria fornix and that the dogma that the hippocampus mediates all forms of spatial learning requires revision.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.102978 |
| Date | January 2006 |
| Creators | Gaskin, Stephane. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Psychology.) |
| Rights | © Stephane Gaskin, 2006 |
| Relation | alephsysno: 002611944, proquestno: AAINR32182, Theses scanned by UMI/ProQuest. |
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