Hemodialysis is widely used for kidney failure patients, it is well known that oxidative stress is induced during hemodialysis process. To figure out what kind of proteins may adhere to the hemodialysis tube, and were those proteins oxidized during dialysis process. In this study, proteins adhere to hemodialysis membrane are eluted and examined by protein two-dimensional gel electrophoresis and MALDI-TOF technique. The two-dimensional gel electrophoresis results demonstrate 153 proteins binding to hemodialysis tube including Vsm-Rho GEF, Fibroblast growth factor 23, Prothrombin, Glomulin and Nucleobindin-1. The oxidation detection shows that some tube binding proteins are oxidized including MAP4K3 protein and Sulfiredoxin-1, and the oxidation level of hemodialysis tube binding proteins are higher then serum proteins. In conclusion, we find out 20 novel proteins which bind to hemodialysis tube and 5 novel proteins which oxidized during hemodialysis. And protein oxidation level was related to inflammation where high CRP levels were detected.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0120110-150951 |
| Date | 20 January 2010 |
| Creators | Ho, Ya-wen |
| Contributors | Hsueh-Wei Chang, Hurng-Wern Huang, Kuang-hung Cheng |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0120110-150951 |
| Rights | campus_withheld, Copyright information available at source archive |
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