The aqueous leaf extract of Rubus suavissimus (Chinese sweet leaf tea) was active in inhibiting angiogenesis, which could be used to suppress adipogenesis and cause weight loss. Following this revelation and approach, the anti-obesity effect was thoroughly investigated in normal and obese rat models.
The investigations began with the preparation of quality-controlled extract. First, the crude extraction methods were examined leading to the characterization of the chemical composition. Second, the impure components were removed by alcohol precipitation and column chromatography, resulting in a standardized sweet leaf tea extract (RUS). Third, three bioavailable compounds identified from the urine analyses were re-constituted to form the purified sweet leaf tea extract (GER), which accounted for 27% by weight of the RUS. These standardized and purified sweet leaf tea extracts were then evaluated for their anti-obesity effect in normal and obese-prone rats.
Both RUS and GER reduced body weight gain by 7% (p =0.999) in normal rodents but statistical analyses failed to show any significance due to small sample size. Amazingly, it was discovered that the use of the tri-compounds combination (GER) produced the same anti-obesity effect as the standardized extract (RUS). Consequently, new experiments were focused on the GER using obese-prone rats as the study model. GER showed a significant anti-obesity effect by reducing 22% (p¡Ü 0.001) weight gain compared to the high-fat diet control group. Total abdominal fat was reduced significantly by 48%, indicating that the lost body mass was mainly due to the loss of body fat. Other benefits of the Chinese sweet leaf tea extract included lowered blood glucose, cholesterol, and triglycerides. Food intake was not affected. No adverse effects were observed.
This research provides exciting insights about the novel use of the Chinese sweet leaf tea as a safe and effective anti-obesity agent beyond its historic use as a natural sweetener. Human clinical investigations are strongly warranted to determine the effective doses and long-term use safety in combating obesity or managing body weight.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-10202009-094300 |
| Date | 22 October 2009 |
| Creators | Koh, Gar Yee |
| Contributors | Liu, Zhijun, Xu, Zhimin, Martin, Roy |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-10202009-094300/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
Page generated in 0.0019 seconds