This study investigated a Soxhlet extract procedure to separate chemical components in the wood and bark of Port-Orford cedar (POC) (Chamaecyparis lawsoniana). The bio-activities, antioxidant properties, and decay resistance were evaluated. The heartwood extracts were analyzed by gas chromatography-mass spectrometry (GC-MS). Three vitro assays including (1) 1, 1-diphenyl-2-picrylhydrazyl hydrate (DPPH ) radical scavenging assay, (2) 2,2-azinobis ( 3-ethylbenzothiszoline-6-sulfonic acid) diammonium salt (ABTS) radical cation scavenging ability assay, and (3) metal chelating activity assay were carried out to evaluate the antioxidant activity. The total phenol content of the extracts was determined by the Folin-Ciocalteu method and expressed as Gallic Acid Equivalent (GAE). The results showed that the methanol extracts of the heartwood and the sapwood contained moderate radical scavenging activities, and the inner bark and outer bark extracts showed the most activities. The sapwood extracts demonstrated the strongest chelating metal capability. Among the four partitions of POC bark, the n-Butanol extracts (n-Bu) showed the highest inhibition effect on ABTS<sup>+</sup> and the highest amount of total phenolic content, followed by ethyl acetate extracts (EA), water soluble components (Wa) and n-hexane extracts (n-He) subtractions. The correlation suggested that phenolic compounds are likely to contribute to the radical scavenging activity of the methanol extracts. There were three major components found in hexane extracts from the heartwood of POC, which were τ-cadinol, azulene, and τmuurolol. Hexadecadinoic acid was found in ethyl acetate extracts. Both the n-hexane and ethyl acetate extracts of the heartwood of POC had excellent growth inhibitory effects on white- and brown-rot fungi. The n-hexane extracts showed a higher antifungal index in this assay system, which was significantly higher than the ethyl acetate extracts.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-06052007-115906 |
| Date | 03 July 2007 |
| Creators | Gao, Heng |
| Contributors | Chung Y. Hse, Todd F. Shupe, Cornelis F.deHoop |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-06052007-115906/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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