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Previous issue date: 2010-08-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Several diseases caused by different pathogen species can affect the cowpea culture,
causing considerable reduction in its productive potential. Among the diseases of large
occurrence are the viral illnesses. The viruses caused by the families Comoviridae and
Potyviridae, represented by the species Cowpea severe mosaic virus (CPSMV) and
Cowpea aphid-borne mosaic virus (CABMV) respectively, are considered of great
importance. Considering the efficiency of the genetic resistance in the control of these
diseases, this work was carried out aiming at the obtenttion and characterization cowpea
plants with resistance to viruses CPSMV and CABMV, in order to obtain new and
essentially derived cultivars. Eight crosses were performed using the line TE 97-309G-9
and the cultivar Patativa as parental donors of resistance to CPSMV and CABMV, and
cultivars BR3-Tracuateua, BRS-Urubuquara, BRS-Novaera, BRS-Guariba and the landrace
Pretinho as recurrent parents. The isolated virus used in the inoculation of F2 and F2RC1
plants were collected in Embrapa Meio-Norte experimental field, and maintained in
differential lines. The purity of the isolated was evaluated and confirmed in the Laboratory
of Fitovirologia, of Biological Institute, in S?o Paulo. Biparental crosses and triple were
made to obtain F1 generations, and then submitted to self pollinated to develop F2 seeds.
These F2 plants were inoculated with the mixture of the viruses CPSMV (sorotype I and II)
and CABMV. Afterward, the resistant F2 plants were backcrossed with the respective
recurrent parent to obtain F1RC1 seeds. The plants of F1RC1 generation were self pollinated
to develop F2RC1 seeds. The F2RC1 plants were also inoculated with the same mixture of
the viruses, as previously mentioned, and resistant plants were characterized. The
following characters were used: number of days to the first flower; pod length; number of
seeds per pod; weight of 100 seeds; seed color; and seed yield per plant. The segregation of
the F2 generation of biparental crosses and triple and the F2RC1 were analyzed to check the
expected frequency of 15 susceptible plants: 1 resistant plant by the ?2 (Qui square) test.
All the F2 biparental crosses and F2RC1 backcrosses were adjusted to the expected
frequency of 15:1. The characters evaluated in parents and F2RC1 were submitted to
statistical analysis. The averages of the resistant plants F2RC1 in each backcross were
compared with the average of respective recurrent parent by the t-test and the averages
within crosses were compared by the Scott-Knott test. The existence of great genetic
variability within and among backcrosses were detected based on all characters. It can be
concluded by this study that all backcrosses are promising for obtaining essentially derived
cultivars resistant to the viruses CPSMV and CABMV and that the backcrosses who
obtained as recurrent parents cultivars BRS-Urubuquara and BRS Novaera are very
promising for obtaining new cultivars with multiple resistance to the viruses CPSMV and
CABMV. / Diversas doen?as causadas por diferentes esp?cies de pat?genos podem afetar a cultura do
feij?o-caupi, promovendo redu??o consider?vel do seu potencial produtivo. Dentre as
doen?as de larga ocorr?ncia se destacam as viroses, merecendo maior ?nfase as causadas
pelos v?rus das fam?lias Comoviridae e Potyviridae, com as esp?cies Cowpea severe
mosaic virus (CPSMV) e Cowpea aphid-borne mosaic virus (CABMV), respectivamente.
Considerando a efici?ncia da resist?ncia gen?tica no controle dessas doen?as, esse trabalho
foi conduzido com o objetivo de obten??o e caracteriza??o de plantas de feij?o-caupi com
resist?ncia aos v?rus CPSMV e CABMV, visando o desenvolvimento de cultivares
essencialmente derivadas e de novas cultivares. Foram realizados oito cruzamentos
utilizando a linhagem TE 97-309G-9 e a cultivar Patativa como parentais doadores dos
genes de resist?ncia aos v?rus CPSMV e CABMV, e as cultivares BR3-Tracuateua, BRSUrubuquara,
BRS-Novaera, BRS-Guariba e a cultivar local Pretinho como parentais
recorrentes. Os isolados virais usados nas inocula??es das plantas das gera??o F2 e F2RC1
foram coletados no campo experimental da Embrapa Meio-Norte, e mantidos em linhagens
diferenciadoras. A pureza dos isolados foi avaliada e confirmada no laborat?rio de
Fitovirologia, do Instituto Biol?gico, em S?o Paulo. Cruzamentos biparentais e cruzamento
triplo foram realizados para a obten??o da gera??o F1 e submetidos ? autofecunda??o para a
obten??o de sementes F2. As plantas da gera??o F2 foram inoculadas com a mistura dos
v?rus CPSMV (sorotipos I e II) e CABMV. Em seguida, as plantas F2 resistentes foram
retrocruzadas com o respectivo parental recorrente para a obten??o de sementes F1RC1. As
plantas da gera??o F1RC1 foram autofecundadas para a obten??o de sementes F2RC1. As
plantas F2RC1 foram tamb?m inoculadas com a mistura dos v?rus, como mencionado
anteriormente e as resistentes foram caracterizadas. Foram avaliados os seguintes
caracteres: n?mero de dias para o in?cio da flora??o; comprimento da vagem; n?mero de
gr?os por vagem; peso de cem gr?os; produ??o de gr?os por planta. As segrega??es das
gera??es F2 dos cruzamentos biparentais e cruzamento triplo e F2RC1 foram analisadas pelo
teste ?2 (Qui-Quadrado) para verificar a freq??ncia esperada de 15 plantas suscet?veis: 1
planta resistente. Todas as F2 dos cruzamentos biparentais e cruzamento triplo e F2RC1
ajustaram-se a freq??ncia esperada de 15:1. Os caracteres avaliados nos parentais e nas
gera??es F2RC1 foram submetidos a an?lise estat?stica. As m?dias das plantas F2RC1
resistentes, de cada retrocruzamento, foram comparadas com a m?dia do seu respectivo
parental recorrente pelo teste t e as m?dias dos retrocruzamentos foram comparadas pelo
teste de Scott-Knott. Foi detectada grande variabilidade gen?tica entre e dentro os
retrocruzamentos para todos os caracteres. Pode-se concluir com base nos resultados que
todos os retrocruzamentos s?o promissores para obten??o de cultivares essencialmente
derivadas resistentes aos v?rus CPSMV e CABMV e que os retrocruzamentos que
obtiveram como parentais recorrentes as cultivares BRS-Urubuquara e BRS Novaera s?o
muito promissores para a obten??o de novas cultivares com resist?ncia aos v?rus CPSMV e
CABMV.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/496 |
| Date | 05 August 2010 |
| Creators | Barros, Gislanne Brito |
| Contributors | Veiga, Carlos Frederico de Menezes |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Fitossanidade e Biotecnologia Aplicada, UFRRJ, Brasil, Entomologia Aplicada |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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