The design and development of a versatile time resolved fluorescence
spectroscopy (TRFS) system capable of near real time data acquisition and processing for
potential clinical diagnostic applications is reported. The TRFS apparatus is portable,
versatile and compatible with the clinical environment. The main excitation source is a
UV nitrogen laser with a nanosecond pulse width and the detection part consists of a dual
grating spectrograph coupled with an MCP-PMT. The nitrogen laser also has a dye
module attached to it, which enables broadband excitation of the sample. This setup
allows rapid acquisition (250 ms for fluorescence decay at a wavelength) of time resolved
fluorescence data with a high spectral (as low as 0.5 nm) and temporal (as low as 25
picoseconds) resolution. Alternatively, a state diode pumped pulsed laser can be used for
excitation to improve data collection speed. The TRFS system is capable of measuring a
broad range of fluorescence emission spectra (visible to near infra-red) and resolving a
broad range of lifetimes (ranging from a few hundred picoseconds to several
microseconds). The optical setup of the system is flexible permitting the connection of
different light sources as well as optical fiber based probes for light delivery/collection
depending on the need of the application. This permits the use of the TRFS apparatus in in vitro, ex vivo and in vivo applications. The system is fully automated for real-time data
acquisition and processing, facilitating near-real time clinical diagnostic applications.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2009-05-795 |
| Date | 2009 May 1900 |
| Creators | Trivedi, Chintan A. |
| Contributors | Jo, Javier A. |
| Source Sets | Texas A and M University |
| Language | English |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | application/pdf |
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