In this doctoral thesis we describe a series of stimuli-responsive polymers which could be used as diagnostic tools or as smart drug delivery systems with simultaneous diagnosis (theranostics). All hereby mentioned polymers are thermoresponsive copolymers of (N-2,2-difluoroethyl)acrylamide exhibiting lower critical solution temperature in aqueous milieu. This means that they are water-soluble at low temperature while when heated above a certain temperature, they self-assemble into nano- or macro- sized assemblies. Because of the high concentration of fluorine atoms, all these polymers could be used as 19 F MRI tracers. We designed multiple different thermoresponsive, thermo- and pH-responsive, thermo-and reactive-oxygen-species-responsive polymers that could find numerous discussed applications in human medicine. We investigated their physico-chemical properties with 1 H and 19 F nuclear magnetic resonance (NMR), size exclusion chromatography (SEC), elemental analysis, dynamic light scattering (DLS), static light scattering (SLS), small angle neutron scattering (SANS), small-angle X-ray scattering (SAXS), and turbidimetry. After the physicochemical optimization of the parameters for the selected applications, their biocompatibility was tested in vitro. Several promising polymers were tested in vivo...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:437211 |
| Date | January 2020 |
| Creators | Kolouchová, Kristýna |
| Contributors | Hrubý, Martin, Matějíček, Pavel, Poučková, Pavla |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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