Wang Zhi-Jun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 54-63). / ABSTRACT --- p.VI / Chapter I. --- INTRODUCTION --- p.1 / Chapter A. --- Literature review --- p.1 / Chapter 1. --- Retinal iron toxicity --- p.2 / Clinical siderotic retinopathy --- p.2 / Experimental siderotic retinopathy --- p.4 / Free radical involvement in siderotic retinopathy --- p.5 / Chapter 2. --- Experimental photic retinopathy in rats --- p.8 / Morphologic features --- p.8 / Free radical involvement in photic retinopathy --- p.9 / Chapter 3. --- Mechanisms of cell death --- p.9 / Necrosis --- p.10 / Apoptosis --- p.10 / Chapter B. --- Statement of the problems --- p.15 / Chapter II. --- MATERIALS AND METHODS --- p.17 / Chapter A. --- Siderotic retinopathy model --- p.17 / Animals --- p.17 / Reagents and equipment --- p.18 / Surgical procedures --- p.18 / Chapter B. --- Histochemical methods --- p.18 / Reagents and equipment --- p.19 / Paraffin sections --- p.19 / H&E staining --- p.19 / TUNEL technique --- p.20 / Schmeltzer's iron staining --- p.21 / Chinoy's ascorbic acid staining --- p.21 / Chapter C. --- Biochemical methods --- p.21 / Reagents and equipment --- p.22 / DNA gel electrophoresis --- p.22 / Analysis of ascorbic acid and uric acid --- p.23 / Chapter III. --- RESULTS --- p.24 / Chapter A. --- Observations in rats --- p.24 / Morphologic changes after H&E staining --- p.24 / Visualization of apoptosis by TUNEL technique --- p.25 / Internucleosomal DNA fragmentation --- p.26 / Negative staining of iron in the ONL --- p.27 / Positive staining of ascorbic acid in the ONL --- p.27 / Chapter B. --- Observations in rabbits --- p.27 / Positive staining of ascorbic acid in all retinal layers --- p.27 / Apoptosis occurred in all retinal layers --- p.28 / Changes of ascorbic acid and uric acid after iron implantatio --- p.28 / Chapter IV. --- DISCUSSION --- p.48 / Chapter V. --- CONCLUSION --- p.53 / References --- p.54
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322027 |
| Date | January 1997 |
| Contributors | Wang, Zhi-Jun., Chinese University of Hong Kong Graduate School. Division of Surgical Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, vii, 63 leaves, [15] leaves of plates : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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