Techniques based on principle of nuclear magnetic resonance (NMR) belong to the most modern methods for studying physical, chemical and biological properties of materials [1]. Their universality predestinates them for application in a wide range of scientific disciplines, e.g. in medicine to study properties of tissues. Advantages of techniques utilizing principle of NMR consist in their noninvasiveness and thoughtfulness to human health or studied material. In addition, no undesirable effects of magnetic force field have been so far proved by research. Objectives of this Diploma Thesis are evaluation of MR images of tissue cultures and determination of protons amount included in them. Theoretic part of the Thesis is devoted to the bases of NMR and provides basic overview of MR methods. The spin echo method (SE) is described in more details, including the process of assessment of technique’s parameters, e.g. general magnetization. Practical part of Diploma Thesis is focused on determination of integral of image intensity of clusters of early somatic embryos. Intensity integrals characterizing number of protons in growing cluster were calculated from MR images of spruce embryos contaminated by lead. The intensity of an image weighted by spin density is proportionate to the number of proton nuclei in the chosen slice. The Thesis describes further evaluation of relaxation time T2 from MR images weighted by spin density. Following part is dealing with determination of diffusion from MR images with application of compensation methods, three-measurement arrangement and presentation of obtained results. Images were processed by use of MATLAB and MAREVISI programs.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:218003 |
| Date | January 2009 |
| Creators | Bidman, Petr |
| Contributors | Šmirg, Ondřej, Gescheidtová, Eva |
| Publisher | Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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