Yuen, Wing Sum. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references (leaves 136-155). / Abstract also in Chinese. / Chapter I --- Title page --- p.i / Chapter II --- Acknowledgements --- p.ii / Chapter III --- Abstract --- p.iii / Chapter IV --- Abstract (Chinese version) --- p.vi / Chapter V --- Table of contents --- p.viii / Chapter VI --- List of abbreviations --- p.xv / Chapter VII --- List of figures --- p.xvi / Chapter Chapter 1 --- General introduction --- p.1 / Chapter Chapter 2 --- Literature review --- p.5 / Chapter 2.1 --- Cystic fibrosis transmembrane conductance regulator in human --- p.5 / Chapter 2.1.1. --- Pathology of cystic fibrosis --- p.5 / Chapter 2.1.2. --- CFTR gene and the encoded protein --- p.6 / Chapter 2.1.3. --- Hypothetical model for CFTR function --- p.7 / Chapter 2.1.4. --- Functions of CFTR --- p.7 / Chapter 2.1.5. --- Regulation of CFTR gene expression --- p.8 / Chapter 2.1.6 --- Regulation of CFTR protein --- p.9 / Chapter 2.1.7. --- Discovery of CFTR homologues in other vertebrates --- p.10 / Chapter 2.2 --- Cystic fibrosis transmembrane conductance regulator in teleosts --- p.10 / Chapter 2.2.1. --- Evidence for the presence of CFTR homologue in teleosts --- p.10 / Chapter 2.2.2. --- Molecular cloning of teleost CFTR genes --- p.11 / Chapter 2.2.3. --- Role of teleost CFTR in osmoregulation --- p.13 / Chapter 2.2.3.1. --- Tissue distribution of CFTR --- p.13 / Chapter 2.2.3.2. --- Changes in CFTR expression in response to ambient salinities --- p.14 / Chapter 2.2.3.3. --- Immunocytochemical studies of CFTR --- p.15 / Chapter 2.2.3.4. --- Regulation of CFTR --- p.17 / Chapter 2.3 --- Osmoregulation in teleosts --- p.19 / Chapter 2.3.1. --- Importance of osmoregulation --- p.19 / Chapter 2.3.2. --- Major components of chloride cells in marine teleosts --- p.20 / Chapter 2.3.2.1. --- Overview --- p.20 / Chapter 2.3.2.2. --- Sodium-potassium adenosine triphosphatase (Na+，K+-ATPase) --- p.21 / Chapter 2.3.2.3. --- Cystic fibrosis transmembrane conductance regulator (CFTR) --- p.22 / Chapter 2.3.2.4. --- Na+/K+/2Cr cotransporter (NKCC) --- p.23 / Chapter 2.3.2.5. --- Potassium (K+) channel --- p.25 / Chapter 2.4 --- Endocrine control of osmoregulation --- p.26 / Chapter 2.4.1. --- Overview --- p.26 / Chapter 2.4.2. --- Growth hormone (GH) and insulin-like growth factor I (IGF-I) --- p.27 / Chapter 2.4.2.1. --- Role of GH in osmoregulation --- p.27 / Chapter 2.4.2.2. --- Mediation through IGF-I --- p.29 / Chapter 2.4.2.3. --- Synergic effect with cortisol --- p.30 / Chapter 2.4.3. --- Prolactin (PRL) --- p.30 / Chapter 2.4.3.1. --- Role of PRL in osmoregulation --- p.30 / Chapter 2.4.3.2. --- Synergic effect with cortisol --- p.33 / Chapter 2.4.4. --- Cortisol --- p.33 / Chapter 2.4.4.1. --- Role of cortisol in osmoregulation --- p.33 / Chapter 2.4.4.2. --- Dual functions of cortisol --- p.34 / Chapter Chapter 3 --- Cloning and tissue distribution of silver sea bream CFTR gene --- p.36 / Chapter 3.1 --- Introduction --- p.36 / Chapter 3.2 --- Materials and methods --- p.38 / Chapter 3.2.1. --- Part A: Cloning of silver sea bream CFTR gene --- p.38 / Chapter 3.2.1.1. --- Fish and culture conditions --- p.38 / Chapter 3.2.1.2. --- Sampling of fish --- p.38 / Chapter 3.2.1.3. --- Preparation of first strand cDNA --- p.38 / Chapter 3.2.1.4. --- Design of primers --- p.39 / Chapter 3.2.1.5. --- Semi-quantitative reverse transcriptase (RT)-PCR --- p.40 / Chapter 3.2.1.6 --- Cloning --- p.41 / Chapter 3.2.2. --- Part B: Tissue distribution of CFTR in silver sea bream --- p.41 / Chapter 3.2.2.1. --- Fish and culture conditions --- p.41 / Chapter 3.2.2.2. --- Tissue sampling --- p.42 / Chapter 3.2.2.3. --- Preparation of first strand cDNA --- p.42 / Chapter 3.2.2.4 --- Design of primers --- p.42 / Chapter 3.2.2.5. --- Semi-quantitative reverse transcriptase (RT)-PCR --- p.43 / Chapter 3.3 --- Results --- p.44 / Chapter 3.3.1. --- Part A: Cloning of silver sea bream CFTR gene --- p.44 / Chapter 3.3.2. --- Part B: Tissue distribution of CFTR in silver sea bream --- p.60 / Chapter 3.4 --- Discussion --- p.62 / Chapter 3.4.1. --- Part A: Cloning of silver sea bream CFTR --- p.62 / Chapter 3.4.2. --- Part B: Tissue distribution of CFTR in silver sea bream --- p.64 / Chapter Chapter 4 --- Effect of salinity on CFTR mRNA expression in gill and posterior intestine of silver sea bream --- p.68 / Chapter 4.1 --- Introduction --- p.68 / Chapter 4.2 --- Materials and methods --- p.70 / Chapter 4.2.1. --- Part A: Effect of long-term exposure to different salinities on CFTR expression --- p.70 / Chapter 4.2.1.1. --- Experimental fish and salinity adaptation --- p.70 / Chapter 4.2.1.2. --- Tissue sampling --- p.70 / Chapter 4.2.1.3. --- Serum ion levels --- p.71 / Chapter 4.2.1.4. --- Preparation of first strand cDNA --- p.71 / Chapter 4.2.1.5. --- Design of primers --- p.71 / Chapter 4.2.1.6. --- Semi-quantitative reverse transcriptase (RT)-PCR --- p.71 / Chapter 4.2.1.7. --- Statistical analysis --- p.72 / Chapter 4.2.2. --- Part B: Effect of abrupt transfer on CFTR expression --- p.73 / Chapter 4.2.2.1. --- Experimental fish --- p.73 / Chapter 4.2.2.2. --- Experimental design --- p.73 / Chapter 4.2.2.2.1 --- Experiment 1: Abrupt transfer from seawater (SW) to 6 ppt --- p.73 / Chapter 4.2.2.2.2. --- Experiment 2: Abrupt transfer from 6 ppt to SW --- p.73 / Chapter 4.2.2.3. --- Tissue sampling --- p.74 / Chapter 4.2.2.4. --- Serum ion levels --- p.74 / Chapter 4.2.2.5. --- Preparation of first strand cDNA --- p.74 / Chapter 4.2.2.6. --- Design of primers --- p.75 / Chapter 4.2.2.7. --- Semi-quantitative reverse transcriptase (RT)-PCR --- p.75 / Chapter 4.2.2.8. --- Statistical analysis --- p.75 / Chapter 4.3 --- Results --- p.76 / Chapter 4.3.1. --- Part A: Effect of long-term exposure to different salinities on CFTR expression --- p.76 / Chapter 4.3.1.1. --- Serum ion levels --- p.76 / Chapter 4.3.1.2. --- CFTR expression in gill --- p.76 / Chapter 4.3.1.3. --- CFTR expression in posterior intestine --- p.76 / Chapter 4.3.2. --- Part B: Effect of abrupt salinity transfer on CFTR expression --- p.83 / Chapter 4.3.2.1. --- Experiment 1: Abrupt transfer from SW to 6 ppt --- p.83 / Chapter 4.3.2.1.1. --- Serum ion levels --- p.83 / Chapter 4.3.2.1.2. --- CFTR in gill --- p.83 / Chapter 4.3.2.1.3. --- CFTR in posterior intestine --- p.83 / Chapter 4.3.2.2. --- Experiment 2: Abrupt transfer from 6 ppt to SW --- p.89 / Chapter 4.3.2.2.1. --- Serum ion levels --- p.89 / Chapter 4.3.2.2.2. --- CFTR in gill --- p.89 / Chapter 4.3.2.2.3. --- CFTR in posterior intestine --- p.89 / Chapter 4.4 --- Discussion --- p.95 / Chapter 4.4.1. --- Long-term exposure to various salinities --- p.95 / Chapter 4.4.2. --- Abrupt salinity transfer --- p.98 / Chapter 4.4.2.1. --- Abrupt hypo-osmotic transfer (33 ppt to 6 ppt) --- p.98 / Chapter 4.4.2.2. --- Abrupt seawater transfer (6 ppt to 33 ppt) --- p.99 / Chapter 4.4.3. --- CFTR mRNA expression in posterior intestine --- p.101 / Chapter 4.4.4. --- Conclusion --- p.101 / Chapter Chapter 5 --- Effect of hormones on CFTR expression in gill and posterior intestine of silver sea bream --- p.102 / Chapter 5.1 --- Introduction --- p.102 / Chapter 5.2 --- Materials and methods --- p.104 / Chapter 5.2.1. --- Part A: In vivo effect of hormones on CFTR expression --- p.104 / Chapter 5.2.1.1. --- Experimental fish and salinity adaptation --- p.104 / Chapter 5.2.1.2. --- Hormone treatment --- p.104 / Chapter 5.2.1.3. --- Tissue sampling --- p.105 / Chapter 5.2.1.4. --- "Serum ion levels, preparation of first strand cDNA, design of primers and semi-quantitative reverse transcriptase (RT)-PCR" --- p.105 / Chapter 5.2.1.5. --- Statistical analysis --- p.105 / Chapter 5.2.2. --- Part B: In vitro effect of hormones on CFTR expression --- p.106 / Chapter 5.2.2.1. --- Fish and culture conditions --- p.106 / Chapter 5.2.2.2. --- Gill and posterior intestine preparations --- p.106 / Chapter 5.2.2.3. --- Hormone treatment --- p.106 / Chapter 5.2.2.4. --- "Preparation of first strand cDNA, design of primers and semi-quantitative reverse transcriptase (RT)-PCR" --- p.107 / Chapter 5.2.2.5. --- Statistical analysis --- p.107 / Chapter 5.3 --- Results --- p.108 / Chapter 5.3.1. --- Part A: In vivo effect of hormones on CFTR expression --- p.108 / Chapter 5.3.1.1. --- Serum ion levels --- p.108 / Chapter 5.3.1.1.1. --- Serum [Na+] level --- p.108 / Chapter 5.3.1.1.2. --- Serum [K+] level --- p.108 / Chapter 5.3.1.1.3. --- Serum [Cl' ] level --- p.108 / Chapter 5.3.1.2. --- CFTR expression in gill --- p.109 / Chapter 5.3.1.3. --- CFTR expression in posterior intestine --- p.109 / Chapter 5.3.2. --- Part B: In vitro effect of hormones on CFTR expression --- p.115 / Chapter 5.3.2.1. --- CFTR expression in gill --- p.115 / Chapter 5.3.2.2. --- CFTR expression in posterior intestine --- p.115 / Chapter 5.4 --- Discussion --- p.122 / Chapter 5.4.1. --- Effects of cortisol on CFTR expression --- p.122 / Chapter 5.4.2. --- Effects of growth hormone on CFTR expression --- p.124 / Chapter 5.4.3. --- Effects of prolactin on CFTR expression --- p.127 / Chapter 5.4.4. --- "Overall effect of cortisol, growth hormone and prolactin on CFTR expression" --- p.128 / Chapter 5.4.5 --- Conclusion --- p.130 / Chapter Chapter 6 --- General discussion and conclusion --- p.132 / References --- p.136
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326727 |
| Date | January 2009 |
| Contributors | Yuen, Wing Sum., Chinese University of Hong Kong Graduate School. Division of Life Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xix, 155 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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