Zhang, Chaoxiong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 115-132). / Abstracts in English and Chinese. / Chapter I --- Title page --- p.i / Chapter II --- Thesis committee --- p.ii / Chapter III --- Abstract --- p.iii / Chapter IV --- Abstract (Chinese version) --- p.v / Chapter V --- Acknowledgement --- p.vii / Chapter VI --- Table of content --- p.viii / Chapter VII --- List of figure --- p.xiii / Chapter Chapter 1 --- General introduction --- p.1 / Chapter Chapter 2 --- Literature review --- p.7 / Chapter 2.1 --- An introduction to myostatin --- p.8 / Chapter 2.1.1 --- A general introduction --- p.8 / Chapter 2.1.2 --- Myostatin identification --- p.9 / Chapter 2.1.3 --- Structural studies of myostatin --- p.10 / Chapter 2.1.4 --- Phenotype of myostatin-null animals or transgenic animal --- p.10 / Chapter 2.2 --- Regulation of myostatin --- p.12 / Chapter 2.2.1 --- Biosynthesis of myostatin --- p.12 / Chapter 2.2.2 --- Regulation of myostatin expression --- p.13 / Chapter 2.2.3 --- Regulation of myostatin protein --- p.16 / Chapter 2.3 --- Myostatin effect --- p.20 / Chapter 2.3.1 --- Myostatin Signaling Pathway --- p.20 / Chapter 2.3.2 --- Cellular Responses to Myostatin Signaling --- p.23 / Chapter 2.4 --- Possible functions in tissues other than muscle --- p.26 / Chapter 2.5 --- Myostatin in fishes --- p.27 / Chapter 2.5.1 --- Introduction of silver sea bream --- p.27 / Chapter 2.5.2 --- Studies carried out in fishes --- p.27 / Chapter 2.5.3 --- Possible novel functions of myostatin in fishes --- p.30 / Chapter Chapter 3 --- Characterization of myostatin gene in the silver seabream (Sparus sarba) --- p.31 / Chapter 3.1 --- Abstract --- p.32 / Chapter 3.2 --- Introduction --- p.33 / Chapter 3.3 --- Materials and methods --- p.35 / Chapter 3.3.1 --- Experimental fish --- p.35 / Chapter 3.3.2 --- Total RNA extraction and cDNA cloning of myostatin-1 and myostatin-2 in silver sea bream --- p.35 / Chapter 3.3.3 --- Multiple sequence alignment --- p.38 / Chapter 3.3.4 --- Real-time PCR for quantification of myostatin-1 and myostatin-2 mRNA expression --- p.38 / Chapter 3.3.5 --- 1 --- p.39 / Chapter 3.3.6 --- Data processing and statistical analysis --- p.40 / Chapter 3.4 --- Results --- p.40 / Chapter 3.4.1 --- Cloning of myostatin-l and myostatin-2 cDNA --- p.40 / Chapter 3.4.2 --- Myostatin tissue distribution and seasonal pattern --- p.42 / Chapter 3.5 --- Discussion --- p.55 / Chapter Chapter 4 --- "Effects of growth hormone, 11-ketotestosterone and cortisol on myostatin mRNA expression in silver sea bream (Sparus sarba)" --- p.61 / Chapter 4.1 --- Abstract --- p.62 / Chapter 4.2 --- Introduction --- p.63 / Chapter 4.3 --- Materials and methods --- p.65 / Chapter 4.3.1 --- Experimental fish --- p.65 / Chapter 4.3.2 --- Growth hormone injection --- p.65 / Chapter 4.3.3 --- 11-ketotestosterone and cortisol injection --- p.66 / Chapter 4.3.4 --- Muscle explants culture and hormone exposure --- p.67 / Chapter 4.3.5 --- Primary pituitary cell culture and cortisol exposure --- p.68 / Chapter 4.3.6 --- Measurement of growth hormone secretion by ELISA --- p.69 / Chapter 4.3.7 --- Data processing and statistical analysis --- p.70 / Chapter 4.4 --- Results --- p.71 / Chapter 4.4.1 --- Growth hormone injection --- p.71 / Chapter 4.4.2 --- 11-ketotestosterone injection --- p.71 / Chapter 4.4.3 --- Cortisol injection --- p.71 / Chapter 4.4.4 --- "In vitro hormone treatment-growth hormone, 11-ketotestosterone and cortisol" --- p.72 / Chapter 4.4.5 --- Pituitary cell growth hormone secretion under cortisol treatment --- p.72 / Chapter 4.5 --- Discussion --- p.81 / Chapter Chapter 5 --- Expression of myostatin mRNA in silver sea bream in different salinity --- p.87 / Chapter 5.1 --- Abstract --- p.88 / Chapter 5.2 --- Introduction --- p.89 / Chapter 5.3 --- Materials and Methods --- p.91 / Chapter 5.3.1 --- Experimental fish --- p.92 / Chapter 5.3.2 --- Long term salinity adaptation --- p.92 / Chapter 5.3.3 --- Abrupt transfer form seawater to freshwater --- p.92 / Chapter 5.3.4 --- Data processing and statistical analysis --- p.93 / Chapter 5.4 --- Results --- p.93 / Chapter 5.4.1 --- Long term adaptation to different salinities --- p.93 / Chapter 5.4.2 --- Abrupt transfer from 33ppt to 6ppt - 24 h --- p.93 / Chapter 5.4.3 --- Abrupt transfer from 33ppt to 6ppt - 72 h --- p.94 / Chapter 5.5 --- Discussion --- p.104 / Chapter Chapter 6 --- General discussion and conclusion --- p.108 / References --- p.115
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327048 |
| Date | January 2010 |
| Contributors | Zhang, Chaoxiong., Chinese University of Hong Kong Graduate School. Division of Life Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiv, 132 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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