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Aetiology of allergic rhinitis in Hong Kong.

by Lit Choi Wan. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 119-127). / ACKNOWLEDGEMENTS / SUMMARY / LIST OF TABLES / LIST OF FIGURES / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter 1.1 --- Terminology & Overview --- p.2 / Chapter 1.2 --- Gell & Coombs Classification of Hypersensitivity --- p.3 / Chapter 1.2.1 --- Type I - Immediate Hypersensitivity --- p.4 / Chapter 1.2.2 --- Type II - Antibody Dependent Cytotoxic Hypersensitivity --- p.4 / Chapter 1.2.3 --- Type III - Immune-Complex Mediated Hypersensitivity --- p.6 / Chapter 1.2.4 --- Type IV - Cell-Mediated (Delayed-Type) Hypersensitivity --- p.6 / Chapter 1.3 --- Historical Review of IgE-Mediated Hypersensitivity --- p.7 / Chapter 1.4 --- The Clinical Biochemistry of IgE --- p.8 / Chapter 1.4.1 --- Structure and Properties --- p.9 / Chapter 1.4.2 --- The Fcε Receptors --- p.11 / Chapter 1.4.3 --- Production and Tissue Localisation --- p.11 / Chapter 1.4.4 --- Turnover in Blood and on Mast Cells --- p.13 / Chapter 1.4.5 --- Control of Synthesis --- p.13 / Chapter 1.4.6 --- The Role of IgE in Health and Disease --- p.14 / Chapter 1.5 --- Pathophysiology of IgE-Mediated Hypersensitivity --- p.20 / Chapter 1.5.1 --- Cell Activation and IgE Synthesis --- p.20 / Chapter 1.5.2 --- Activation of Mast Cells and Basophils --- p.22 / Chapter 1.6 --- Allergens --- p.25 / Chapter 1.7 --- Factors Predisposing to Allergy --- p.30 / Chapter 1.7.1 --- Genetic Predisposition --- p.30 / Chapter 1.7.2 --- Environmental Exposure --- p.32 / Chapter 1.7.3 --- Underlying Infection and Immune Status --- p.32 / Chapter 1.8 --- Epidemiology --- p.33 / Chapter 1.8.1 --- Allergic Diseases Worldwide --- p.33 / Chapter 1.8.2 --- Allergic Diseases in Hong Kong --- p.34 / Chapter 1.9 --- Diagnosis of IgE-Mediated Allergy --- p.36 / Chapter 1.9.1 --- Medical History and Physical Examination --- p.36 / Chapter 1.9.2 --- Introduction to Diagnostic Tests --- p.36 / Chapter 1.9.3 --- Skin Tests --- p.37 / Chapter 1.9.4 --- In Vitro Tests --- p.38 / Chapter 1.9.5 --- Serum Total IgE Assay --- p.41 / Chapter (1) --- Test Principle --- p.41 / Chapter (2) --- Normal Values of Serum Total IgE Concentration --- p.42 / Chapter 1.9.6 --- Allergen Specific IgE Concentration --- p.44 / Chapter (1) --- Principle of RAST --- p.44 / Chapter (2) --- Clinical Usefulness of RAST --- p.44 / Chapter 1.9.7 --- The CAP System --- p.46 / Chapter 1.9.8 --- Mixed Aeroallergen Specific IgE Assay (Phadiatop´ёØ) --- p.46 / Chapter 1.10 --- Further Notes on Allergic Rhinitis --- p.48 / Chapter 1.10.1 --- A Prevalent and Costly Health Problem --- p.48 / Chapter 1.10.2 --- Epidemiology --- p.48 / Chapter 1.10.3 --- Symptoms of Allergic Rhinitis --- p.49 / Chapter 1.10.4 --- ENT Examination --- p.49 / Chapter 1.11 --- Aims of This Study --- p.51 / Chapter CHAPTER 2 --- MATERIALS AND METHOD I Evaluation of The Pharmacia CAP System --- p.52 / Chapter 2.1 --- Materials --- p.53 / Chapter 2.1.1 --- Pharmacia Reagents --- p.53 / Chapter 2.1.2 --- Abbott Reagents --- p.54 / Chapter 2.1.3 --- In-House Reagents --- p.54 / Chapter 2.2 --- Apparatus and Equipment --- p.55 / Chapter 2.2.1 --- Components and Accessories of the CAP System --- p.55 / Chapter 2.2.2 --- Abbott IMx Analyser --- p.55 / Chapter 2.3 --- Methods for Evaluation of the Pharmacia CAP System --- p.56 / Chapter 2.3.1 --- Principle of the Pharmacia CAP System RAST FEIA Fluorescence Enzyme Immunoassay --- p.56 / Chapter 2.3.2 --- Assay Preparation and Procedure --- p.58 / Chapter 2.3.3 --- Evaluation Experiments --- p.59 / Chapter (1) --- Elution Efficiency of Assay Washer96 --- p.59 / Chapter - --- Gross Assessment of Assay Washer96 --- p.59 / Chapter - --- Precision of Elution Volume in Each Well --- p.60 / Chapter - --- Overall Precision and Accuracy of Elution through ImmunoCAP --- p.60 / Chapter (2) --- Precision of RAST FEIA --- p.60 / Chapter (3) --- Accuracy --- p.61 / Chapter (4) --- Linearity and Detection Limits --- p.61 / Chapter (5) --- Recovery Study --- p.61 / Chapter CHAPTER 3 MATERIALS --- AND METHOD II Aetiology of Allergic Rhinitis in Hong Kong --- p.62 / Chapter 3.1 --- Patients and Controls --- p.63 / Chapter 3.2 --- Blood Samples --- p.63 / Chapter 3.3 --- Serum Total IgE Assay --- p.65 / Chapter 3.4 --- Mixed Aeroallergen Specific IgE (Phadiatop) --- p.67 / Chapter 3.5 --- Statistical Analysis --- p.67 / Chapter CHAPTER 4 --- RESULTS I Evaluation of The Pharmacia CAP System --- p.70 / Chapter 4.1 --- Elution Efficiency of Assay Washer96 --- p.71 / Chapter 4.1.1 --- Gross Assessment of Elution Volume --- p.71 / Chapter 4.1.2 --- Precision of Elution Volume in Each Well --- p.72 / Chapter 4.1.3 --- Overall Precision and Accuracy of Elution through ImmunoCAP --- p.75 / Chapter 4.2 --- Precision of RAST FEIA --- p.75 / Chapter 4.3 --- Accuracy --- p.75 / Chapter 4.4 --- Linearity and Detection Limit --- p.79 / Chapter 4.5 --- Recovery --- p.79 / Chapter CHAPTER 5 --- RESULTS II Aetiology of Allergic Rhinitis in Hong Kong --- p.83 / Chapter 5.1 --- Patients and Controls --- p.84 / Chapter 5.2 --- "Serum Total IgE, Mixed Aeroallergen IgE (Phadiatop) and Specific IgE Concentrations in Patients and Controls" --- p.84 / Chapter 5.2.1 --- Total IgE Concentration --- p.84 / Chapter 5.2.2 --- Phadiatop´ёØ --- p.84 / Chapter 5.2.3 --- Specific IgE Concentration --- p.93 / Chapter 5.2.4 --- Degree and Severity of Sensitization --- p.93 / Chapter 5.2.5 --- Single and Multiple Allergy --- p.94 / Chapter 5.2.6 --- Usefulness of Phadiatop´ёØ --- p.94 / Chapter CHAPTER 6 --- DISCUSSION I Evaluation of the Pharmacia CAP System --- p.95 / Chapter 6.1 --- Elution Efficiency of Assay Washer96 --- p.96 / Chapter 6.2 --- "Within-Batch and Between-Batch Precision, Accuracy, Linearity and Detection Limit, and Recovery of FEIA" --- p.97 / Chapter 6.3 --- Overall Evaluation --- p.98 / Chapter CHAPTER 7 --- DISCUSSION II Aetiology of Allergic Rhinitis in Hong Kong --- p.100 / Chapter 7.1 --- Patients and Controls --- p.101 / Chapter 7.2 --- Serum Total IgE Concentration --- p.102 / Chapter 7.3 --- Efficiency of Phadiatop´ёØ Screening Test --- p.103 / Chapter 7.4 --- Serum Specific IgE Assay and Aetiology of Allergic Rhinitis in Hong Kong --- p.107 / Chapter 7.4.1 --- Major Allergens in Allergic Rhinitis --- p.107 / Chapter 7.4.2 --- House Dust Mite --- p.108 / Chapter 7.4.3 --- Cockroach --- p.111 / Chapter 7.4.4 --- Cat and Dog --- p.111 / Chapter 7.4.5 --- Pollens --- p.112 / Chapter 7.4.6 --- Moulds --- p.113 / Chapter 7.4.7 --- Summary --- p.115 / REFERENCES --- p.118

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321507
Date January 1996
ContributorsLit, Choi Wan., Chinese University of Hong Kong Graduate School. Division of Clinical Biochemistry.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiii, 127 leaves : ill. (some mounted col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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