A total of 43 Rhizobium leguminosarium bv. trifolii isolates were obtained from
soil samples of two ball clover (Trifolium nigrescens) pastures from Iola and Kilgore
(Texas) using ball clover as capture plants. The isolates were phenotypically
characterized by intrinsic antibiotic resistance (IAR) against a range of concentrations of
eight antibiotics, and by the utilization of 95 different carbon sources (BIOLOG system).
The rhizobial isolates were also evaluated for their tolerance to salinity, high
temperatures and low pH. The isolates showed two different ranges of growth rates:
fast-growing (doubling times between 1.4 - 3.7 h) and slow- growing isolates (12.3 -
21.3 h). The numerical analysis of the phenotypic characteristics showed that the 43
isolates could be grouped in 24 different strains. Cluster analysis based on sensitivity
responses of IAR, metabolic profiles of BIOLOG and salt, temperature and acidity
tolerance levels could distinguish the Rhizobium strains from a Pseudomonas isolate.
The analysis also showed that the rhizobial strains isolated from ball clover nodules are
different from a commercial R. leguminosarium bv. trifolii strain used as inoculant for
this legume.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/2636 |
| Date | 01 November 2005 |
| Creators | Cepeda Hernandez, Martha Lucia |
| Contributors | Evers, Gerald W., Weaver, Richard W. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | 654362 bytes, electronic, application/pdf, born digital |
Page generated in 0.0018 seconds