Sze, Wing Ho Angel. / "December 2010." / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 90-112). / Abstracts in English and Chinese. / Thesis committee --- p.i / Statement --- p.ii / Acknowledgements --- p.iii / Abstract --- p.iv / 摘要 --- p.vi / Table of Contents --- p.viii / List of Tables --- p.xii / List of Figures --- p.xiii / List of Abbreviations --- p.xv / Chapter Chapter 1. --- General Introduction --- p.1 / Chapter Chapter 2. --- Literature Review / Chapter 2.1. --- Carotenoids in plants --- p.5 / Chapter 2.2 --- Carotenoid biosynthesis in plants --- p.7 / Chapter 2.3. --- Carotenoids in animals --- p.11 / Chapter 2.4. --- Vitamin A deficiency (VAD) --- p.13 / Chapter 2.5. --- Recommended requirement of vitamin A --- p.15 / Chapter 2.6. --- Bioavailability and bioconversion of dietary carotenoids --- p.17 / Chapter 2.7. --- Efforts to improve carotenoid contents in food crops --- p.19 / Chapter 2.8. --- Carotenoid catabolism --- p.20 / Chapter 2.9. --- Carotenoid cleavage dioxygenase (CCD) --- p.21 / Chapter 2.10. --- Carotenoid-derived phytohormones --- p.24 / Chapter 2.11. --- "CCD and carotenoid-derived colors, aromas and flavors" --- p.27 / Chapter Chapter 3. --- Hypothesis and Objectives --- p.35 / Chapter Chapter 4. --- Materials and methods / Chapter 4.1. --- General cloning and sequencing --- p.36 / Chapter 4.2. --- Extraction of RNA and DNase treatment --- p.36 / Chapter 4.3. --- Reverse transcription --- p.37 / Chapter 4.4. --- Real-time quantitative RT-PCR --- p.39 / Chapter 4.5. --- Cloning of OsCCD1 cDNA --- p.40 / Chapter 4.6. --- Bacterial in vivo assay of OsCCD 1 activity --- p.41 / Chapter 4.7. --- Construction of OsCCD1 RNAi constructs --- p.42 / Chapter 4.8. --- "Construction of ""Super-Golden"" rice constructs" --- p.46 / Chapter 4.8.1. --- "Construction of ""GluC-Y1-Nos"" cassette" --- p.46 / Chapter 4.8.2. --- "Construction of ""Gt1-TCN"" cassette" --- p.46 / Chapter 4.8.3. --- "Construction of""pGT-PCC""" --- p.47 / Chapter 4.8.4. --- "Construction of ""pGYGC""" --- p.47 / Chapter 4.9. --- Rice transformation --- p.54 / Chapter 4.10. --- Detection of transgene --- p.57 / Chapter 4.10.1. --- Southern blot --- p.57 / Chapter 4.10.2. --- HPLC analysis of carotenoids in seeds --- p.59 / Chapter Chapter 5. --- Results / Chapter 5.1. --- Expression profiles of carotenogenic genes in rice endosperms --- p.62 / Chapter 5.2. --- Expression of CCDs in developing rice seeds --- p.64 / Chapter 5.3. --- Features of OsCCD1 --- p.68 / Chapter 5.4. --- Characterization of OsCCD1-knock down transgenic rice --- p.72 / Chapter 5.5. --- "Construction of ""Super-Golden"" rice" --- p.78 / Chapter 5.6. --- Phenotypic characterization of PCC transgenic rice --- p.79 / Chapter 5.7. --- HPLC analysis on seed carotenoid content --- p.80 / Chapter Chapter 6. --- Discussion --- p.82 / Chapter Chapter 7. --- Conclusion --- p.89 / References --- p.90
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327422 |
| Date | January 2011 |
| Contributors | Sze, Wing Ho Angel., Chinese University of Hong Kong Graduate School. Division of Life Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xv, 112 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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