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Previous issue date: 2009 / Amostras sanguineas de 188 touros bubalinos, criados em sistema extensivo em areas de varzea, sem controle sanitario e reprodutivo eficiente, com idade de dois a 15 anos,
das racas Murrah, Mediterraneo e seus mesticos, foram coletadas em tubos vacutainer
esterilizados de 5 mL e avaliadas quanto a presenca de anticorpos para HBV-1, atraves
do teste de soroneutrlizacao. Dos 188 touros examinados, somente de 51 foi possivel
obter amostras de semen, as quais foram submetidas a analise de RT-PCR. Informacoes
acerca da idade, raca e escore de condicao corporal (ECC) dos animais foram
observadas. A estatistica descritiva foi aplicada atraves da distribuicao proporcional e
determinacao dos quadris e medianas de variaveis categoricas. O estudo foi realizado
em animais de oito propriedades, localizadas nos estados do Para e Amapa, regiao Norte
do Brasil, encontrando-se distribuidos da seguinte forma: nos municipios de Itaubal do
Piriri (N=59), Cutias do Araguari (N=33), Tartarugalzinho (N=14) e Bailique, distrito
de Macapa (N=21), totalizando 127 animais no Amapa. Os 73 restantes localizavam-se
no estado do Para nos municipios de Soure (N=38) e Muana (N=23), Ilha do Marajo. A
analise estatistica constou da aplicacao do teste Binomial para comparar a prevalencia
do HBV-1 nas propriedades examinadas nos municipios, e comparar a prevalencia entre
as classes de raca, escore corporal e idade. Para avaliar a distribuicao da titulacao de
anticorpos para HBV-1, entre Amapa e Marajo, foi utilizado o teste estatistico de Mann-
Whitney. Todos os procedimentos foram executados pelo programa BioEstat 5, com
nivel de significancia α=0.05. Em todas as propriedades estudadas havia animais
positivos, e 82,4% das 188 amostras foram sorologicamente positivas. Entre as racas
mediterraneo e Murrah e seus mesticos, houve diferenca real de prevalencia somente
entre as racas Mediterraneo e Murrah (p=0,0004). Houve influencia do ECC na
prevalencia de touros sorologicamente positivos, sendo que os de ECC=3 apresentaram
maior prevalencia que os de ECC < 3 (p=0.009). A prevalencia conforme a idade
mostrou que existe uma diferenca significativa (p<0.0001) entre os animais com dois
anos e os touros com mais de dois anos de idade, havendo um incremento gradativo das
taxas de infeccao com o avancar da idade. A prevalencia no estado do Amapa foi
significativamente maior que no Para. As propriedades localizadas nos municipios de
Cutias do Araguari, Tartarugalzinho e Bailique (Macapa), apresentram 100% de
prevalencia. Foi observado que a titulacao minima ocorreu na maioria dos animais no
Marajo, entretanto em somente em 4.8% do rebanho procedente do Amapa teve baixas
titulacoes. Todas as 51 amostras de semen apresentaram-se negativas no teste da RTPCR.
Concluimos assim que a prevalencia da IBR e altissima em touros bubalinos
criados extensivamente no estado do Amapa e Para, Brasil. / Blood samples of 188 buffalo bulls, reared in extensive systems in pasture of lowland,
without effective control and reproductive and health, with two to 15 years old, Murrah,
Mediterranean breeds and crossbreeds, were collected in sterile Vacutainer tubes, 5 mL
and analyzed for the presence of antibodies to HBV-1, through the test virus
neutralization. Of the 188 bulls examined, only 51 were obtained from semen of
samples, which were submitted to analysis RT-PCR. Information about age, race and
body condition score (BCS) of the animals were observed. The descriptive statistics was
applied through the proportional distribution and determination of the hips and medians
for categorical variables. The study was conducted on eight properties, located in the
states of Para and Amapa, northern Brazil, were distributed as follows: 127 animals in
the municipalities Itaubal of Piriri (N = 59), Agouti Araguari (N = 33), Tartarugalzinho
(N = 14) and Bailique, district of Macapa (N = 21) located in the state of Amapa. The
remaining 73 located in the state of Para in the municipalities of Soure (N = 38) and
Muana (N = 23), Marajo of Island. Statistical analysis consisted of applying the
Binomial test to compare the prevalence of HBV-1 in the properties examined in the
cities, and compare the prevalence between the classes of race, age and body score. To
assess the distribution of titers of antibodies to HBV-1, between Amapa and Marajo, we
used the statistical test of Mann-Whitney. All procedures were performed by the
program BioEstat 5, with significance level α= 0.05. All studied properties had
seropositive animals, and 82.4% of 188 samples were serologically positive. Between
the Mediterranean and Murrah breeds and crossbreeds, there was only real difference in
prevalence between the Mediterranean and Murrah breeds (p = 0.0004). There was
influence of BCS in the serologically positive bulls prevalence. The BCS = 3 had the
highest prevalence of ECC <3 (p = 0.009). The prevalence according to age showed a
significant difference (p <0.0001) between animals with two years and the bulls with
more than two years of age, with a gradual increase in rates of infection with age. The
prevalence in the state of Amapa was significantly higher than in Para. The properties
located in the municipalities of the Cutias do Araguari, Tartarugalzinho and Bailique
(Macapa), had a 100% prevalence. It was observed that the titration was minimal in
most animals in Marajo, but only 4.8% of the Amapa’s herds was founded low titers.
All 51 samples of semen, were negative in the RT-PCR test. We conclude that the
prevalence of IBR is high in buffalo bulls bred extensively in the state of Para and
Amapa, Brazil.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufpa.br:2011/5221 |
| Date | 28 November 2009 |
| Creators | FERREIRA, Rafaela Nunes |
| Contributors | RIBEIRO, Haroldo Francisco Lobato |
| Publisher | Universidade Federal do Pará, Empresa Brasileira de Pesquisa Agropecuária, Universidade Federal Rural da Amazônia, Programa de Pós-Graduação em Ciência Animal, UFPA, EMBRAPA, UFRA, Brasil, Campus Universitário de Castanhal |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFPA, instname:Universidade Federal do Pará, instacron:UFPA |
| Rights | info:eu-repo/semantics/openAccess |
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