Return to search

The Effects of 3-Deazaguanine on Chick Embryo Fibroblasts and Rat Kidney Cells Infected with Temperature-Sensitive Mutant and Wild-Type Rous Sarcoma Viruses

Chick embryo fibroblasts and rat kidney cells infected in vitro with Rous Sarcoma viruses were treated with 3-deazaguanine (3-DG). The findings revealed that 3-DG inhibited virus-induced cellular transformation. Degree of inhibition is dependent on concentration and frequency of media change. 3-DG at the concentrations tested will not reverse transformed cells to the untransformed state and does not have marked effect on replication of viruses. Upon removal of 3-DG, its effect was shown to be reversible. Cell growth was generally retarded in medium containing 3-DG. When xanthosine and inosine were added to the medium, cell growth was unaffected, but it increased in guanosine.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc504461
Date12 1900
CreatorsFadare, Samuel O.
ContributorsChen, Young C., Hatten, Betty A., Vela, G. Roland, 1927-
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatv, 55 leaves: ill., Text
RightsPublic, Fadare, Samuel O., Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

Page generated in 0.0016 seconds