Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-08-18T12:40:09Z
No. of bitstreams: 1
2016 - Gabriel Almeida Dutra.pdf: 941212 bytes, checksum: 83615886b763ec9a1fb70714c85ae122 (MD5) / Made available in DSpace on 2017-08-18T12:40:09Z (GMT). No. of bitstreams: 1
2016 - Gabriel Almeida Dutra.pdf: 941212 bytes, checksum: 83615886b763ec9a1fb70714c85ae122 (MD5)
Previous issue date: 2016-03-31 / CAPES / The anticipation of the breeding season has been the subject of great interest in front of their
economic impact. In order to advance the first ovulation and anticipate the reproductive phase
of the mares, this study aimed to evaluate if the transvaginal follicle aspiration technique
guided by ultrasound during spring transition period, was able to induce cyclicity in mares.
The experiment was conducted in Animal Reproduction area of DRAA / IZ / UFRRJ, located
at Serop?dica-Rj, during spring transition period (August-September) and early reproductive
period (October) 2015. Selected mares were randomly distributed into three groups: Group 1
Control (G1, n = 9), without hormonal treatment; Group 2 P4 + PGF2? (G2, n = 9), 1500 mg
of long-acting progesterone, intramuscularly (IM), and seven days later, 7.5 mg Dinoprost
(PGF2?), IM and Group 3 transvaginal follicular aspiration + PGF2? (G3, n = 9), greater
follicle aspirated (>25mm) and seven days later, 7.5 mg Dinoprost (PGF2?), IM. Prior to the
beginning of the experiment, all mares were evaluated by ultrasonography, and the criteria
for the study was absence of corpora lutea as well as the presence of ovarian follicles larger
than 25 mm. Subsequent to this evaluation, the reproductive activity was monitored every 48
hours until the second ovulation of each mare. When evaluated the number of mares ovulating
within 16 days after initiation of treatment, it was observed that the groups G2 and G3 were
significantly more effective (P = 0.0031) in the first ovulation of the breeding season, when
compared to G1. In G1, 16 days after the start of treatment, no animal ovulated. In G2 and G3
eigh (88.9%) and six (66.7%) mares ovulated respectively. The mares were evaluated until the
second subsequent ovulation treatment. All mares ovulated normally, showing that none of
them returned to the transitional period and the average number of days between the first and
second ovulation did not differ between the groups (P> 0.05). The results of this study allow
us to conclude that follicular aspiration technique during spring transition period associated
with the administration of 7.5 mg of PGF2? seven days later, was able to induce cyclicity in
XI
mares. This treatment was effective as the conventional protocol P4 + PGF2?, thus possible to
use it in routine reproductive centers. However, more studies are needed to evaluate its
effectiveness during the early spring transition period, as well as its association with ovulation
inducers. / A antecipa??o da esta??o reprodutiva tem sido assunto de grande interesse frente ao seu
impacto econ?mico. A fim de adiantar a primeira ovula??o e antecipar a fase reprodutiva das
?guas, o presente estudo teve como objetivo avaliar se a t?cnica de aspira??o folicular
transvaginal guiada por ultrassom durante o per?odo de transi??o primaveril foi capaz de
induzir a ciclicidade em ?guas. O experimento foi realizado na ?rea de Reprodu??o Animal do
DRAA/IZ/UFRRJ, localizado no munic?pio de Serop?dica-RJ, durante o per?odo de transi??o
primaveril (agosto-setembro) e in?cio do per?odo reprodutivo (outubro) de 2015. Foram
selecionadas 27 ?guas da ra?a Mangalarga Marchador, entre 5-12 anos, pesando entre 350-
450 kg e com hist?rico de atividade reprodutiva normal. As ?guas selecionadas foram
distribu?das aleatoriamente em tr?s grupos: Grupo 1- Controle (G1; n=9), sem tratamento
hormonal; Grupo 2- P4 + PGF2? (G2; n=9), 1500mg de progesterona de longa a??o, por via
intramuscular (IM), e sete dias ap?s, 7,5 mg de Dinoprost (PGF2?), IM e Grupo 3- Aspira??o
folicular transvaginal + PGF2? (G3; n=9), maior fol?culo aspirado (>25mm) e sete dias ap?s,
7,5 mg de PGF2?, IM. Previamente ao in?cio do experimento, todas as ?guas foram avaliadas
por meio da ultrassonografia, sendo crit?rio para o estudo a aus?ncia de corpo l?teo, bem
como a presen?a de fol?culos ovarianos >25mm. Posteriormente a esta avalia??o, a atividade
reprodutiva foi monitorada a cada 48 horas at? o momento da segunda ovula??o de cada ?gua.
Quando avaliado o n?mero de ?guas que ovularam em at? 16 dias ap?s o in?cio do tratamento,
observou-se que os grupos G2 e G3 foram mais eficientes (P=0,0031) em acelerar a primeira
ovula??o da esta??o reprodutiva, quando comparados ao grupo G1. No grupo G1, ap?s 16
dias do in?cio do tratamento, nenhum animal ovulou. Nos grupos G2 e G3 oito (88,9%) e seis
(66,7%) ?guas ovularam, respectivamente. Analisando o n?mero m?dio de dias a partir do
tratamento at? a primeira ovula??o, os grupos tratados (G2 e G3) foram mais eficientes que o
IX
grupo controle na antecipa??o da primeira ovula??o (P<0,001). Em rela??o aos grupos
tratados, n?o houve diferen?a (P>0,05) entre eles. Foi poss?vel verificar que os grupos G2 e
G3 apresentaram uma antecipa??o da primeira ovula??o em rela??o ao controle em m?dia de
12,2 e 8,9 dias, respectivamente. As ?guas foram acompanhadas at? a segunda ovula??o
subsequente ao tratamento. Todas ovularam normalmente, demonstrando que nenhuma delas
retornou ao per?odo de transi??o e que a m?dia de dias entre a primeira e a segunda ovula??o,
n?o diferiu entre os grupos (P>0,05). Os resultados do presente estudo nos permitem concluir
que a t?cnica de aspira??o folicular durante o per?odo de transi??o primaveril, associado ?
administra??o de 7,5 mg de PGF2? sete dias mais tarde, foi capaz de induzir a ciclicidade em
?guas. Este tratamento mostrou-se t?o eficaz quanto o protocolo convencional de P4 + PGF2?,
sendo vi?vel utiliza-lo na rotina de centros reprodutivos. No entanto, mais estudos s?o
necess?rios a fim de avaliar sua efic?cia em fol?culos com di?metros menores no per?odo de
transi??o primaveril, assim como sua associa??o aos indutores de ovula??o.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1972 |
| Date | 31 March 2016 |
| Creators | DUTRA, Gabriel Almeida |
| Contributors | Jacob, Albuquerque, Flamarion Ten?rio de, Mello, Marco Roberto Bourg de |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas), UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | ALJARRAH, A. H. Methods to induce earlier onset of cyclicity in transitional mares. 2004, 65p. Thesis (Master of Science). Jordan University of Science and Technology, Jordan, 2004. ALVARENGA, M. A., et al. Effect of follicular aspiration on ovarian function intransitional mares. Theriogenology, v. 51, n. 1, 1999. AMIRIDIS, G.S., et al. Luteal stage dependence of pituitary response to gonadotrophin-releasing hormone in cyclic dairy ewes subjected to synchronisation of ovulation. Reproduction, Fertility and Development, p.769-774, 2006. BERGFELT, D.R. Anatomy and physiology of the mare. In: SAMPER, J.C. 2ed. Equine breeding management and artificial insemination. Missouri:Saunders Elsevier,113-131p. 2009. BERGFELT, D.R., et al. Response of estradiol and inhibin to experimentally reduced luteinizing hormone during follicle deviation in mares. Biology Reproduction, p.426-432, 2001. BOGH, I.B., et al. Steroid concentration in follicular fluid aspirated repeatedly from transitional and cyclic mares. Theriogenology, p. 877-888, 2000 BOGH, I.B., et al. Ovarian function and morphology in the mare after multiple follicular punctures. Equine Veterinary Journal, p.575-579, 2003. BOTELHO, J.H.V. Indu??o hormonal de estro regular em ?guas Mangalarga Marchador em transi??o primaveril. 2012, 39p. Disserta??o (Mestrado) Universidade Federal de Lavras. Lavras- MG, 2012. BOTELHO, J.H.V., et al. Hormone supplementation protocol using estradiol benzoate and long-acting progesterone is efficient in maintaining pregnancy of anovulatory recipient mares during autumn transitional phase Animal Reproduction Science, p. 39?43, 2015. 24 BRACHER, V., et al. Repeated transvaginal ultrasound-guided follicular aspiration in the mare. Equine Veterinary Journal, p. 75-78, 1993. BRUCK, I., et al. Follicle aspiration in the mare using a transvaginal ultrasoundguided technique. Equine Veterinary Journal, p. 58-59, 1992. BURATINI JR. J. Avalia??o da din?mica folicular em ?guas da ra?a Mangalarga Marchador utilizando a ultrasonografia e as concentra??es plasm?ticas de progesterona e horm?nio luteinizante. Disserta??o de mestrado, Universidade Estadual Paulista UNESP-Botucatu, p. 27-39, 1997. CARNEVALE, E.M. Oocyte transfer and gameta intrafallopian transfer in the mare. Animal Reproduction Science, p. 617-624, 2004. CHRISTENSON & DEVOTO. Cholesterol transport and steroidogenesis by the corpus luteum. Reproductive Biology and Endocrinology, p.1-9, 2003. CUERVO-ARANGO & CLARK. The first ovulation of the breeding season in the mare: the effect of progesterone priming on pregnancy rate and breeding management (hCG response rate and number of services per cycle and mare). Animal Reproduction Science, 2010. DELLENBACH, P., et al. Transvaginal, sonographically controlled ovarian follicle puncture for egg retrieval. Lancet,1984. Diaz F.J., et al. Regulation of progesterone and prostaglandin F2? production in the CL. Molecular and Cellular Endocrinology, p.65-68, 2002. DIELEMAN, S.J., et al. Changes in oestradiol. Progesterone and testosterone concentrations in follicular fluid and in micromorphology of preovulatory bovine follicles relative to the peak of luteinizing hormone. Journal of Endocrinology, p.31-42, 1983. DONADEU, F.X., et al. Effect of number and diameter of follicles on secretion of inhibit and suppression of FSH in mares. Reproduction, p. 897? 903, 2001. 25 DONADEU & WATSON. Seasonal changes in ovarian activity: Lessons learnt from the horse. Animal Reproduction Science, p. 225?242, 2007. DONADEU, F.X., et al. Supression of circulating concentrations of FSH and LH by inhibin and estradiol during the initiation of follicle deviation in mares. Theriogenology, p.1423?1434, 2003. EVANS, A.C.O. Characteristics of ovarian follicle development in domestic animals. Reproduction in Domestic Animals, p. 240 ? 246, 2003. FERREIRA-DIAS, G., et al. Proliferative processes within the equine corpus luteum may depend on paracrine progesterone actions. Journal of Physiology and Pharmacology, p.139-151, 2006. FRASER, H.M., et al. Effects of LH-releasing hormone antagonist on the secretion of LH, FSH, prolactin and ovarian steroids at different stages of the luteal phase in the stumptailed macaque (Macaca arctoides). Journal of Endocrinology, p.83-90, 1986. GASTAL, E.L., et al. Ultrasound-guided intrafollicular treatment in mares. Theriogenology, p.1027-1037, 1995. GASTAL, E.L., et al. Role of diameter differences among follicles in selection of a future dominant follicle in mares. Biology of Reproduction, p.1320-1327, 1997 GASTAL, E. L., et al. Temporal interrelationships among luteolysis, FSH and LH concentrations and follicle deviation in mares. Theriogenology, p. 925 ? 940, 2000. GINTHER, O. J. Reproductive Biology of the Mare: basics and applied aspects. 2? ed. Michigan, U.S.A: EquiservicesPublishing, 1992. GINTHER, O.J. Major and minor follicular waves during the equine estrous cycle. Journal of Equine Veterinary Science, p.18-25, 1993. GINTHER, O. J. Selection of the dominant follicle in cattle and horses. Animal Reproduction Science, p. 60-79, 2000. 26 GINTHER, O. J., et al. Mechanism of follicle deviation in monovular farm species. Animal Reproduction Science, p. 239-257, 2003. GINTHER, O.J., et al. Follicle dynamics and selection in mares. Reproduction, p. 45-63, 2004. GINTHER, O.J, et al. Regulation of circulating gonadotropins by the negative effects of ovarian hormones in mares. Biology of Reproduction, p.315-323, 2005. GORETTI, R.G., et al. Effects of timing of induced luteolysis in embryo donor mares on reproductive performance and pregnancy rate in recipient mares Theriogenology, p. 1170?1174, 2011. GUILLAUME, D., et al. Determination of minimum light treatment required for photostimulation of winter anoestrous mares. Journal of Reproduction and Fertility Supplement, p.205-216, 2000. HAYNA, J.T., et al. The effect of transvaginal follicular aspiration on Corpus luteum formation in mares. Havemeyer Foundation Workshop. In: International symposium on equine embryo transfer. RJ, Brazil, p. 35, 2004. HINRICHS, K., et al. Embryonic development after intrafollicular transfer of horse oocytes. Journal of Reproduction and Fertility, p. 369-374, 1991. JACOB, J.C.F. Din?mica ovariana e end?crina de ?guas em diferentes idades. 2008, 62p. Tese (Doutorado) Universidade Federal de Vi?osa- MG. Vi?osa- MG, 2008. JACOB, J.C., et al. Temporal Relationships and Repeatability of Follicle Diameters and Hormone Concentrations within Individuals in Mares. Reproduction in Domestic Animals, p. 92?99, 2009. 27 LeBLANC, M. Equine stud farm medicine and surgery. In: KNOTTENBELT, D.C. (Ed.). The mare. Hardcover: Saunders, p.124, 2003. LIMA, W. M., et al. Improved superovulatory response in beef cattle following ovarian follicular ablation using a simplified transvaginal device. Animal Reproduction Science, p. 364-70, 2007. MACHADO, M.S. Avalia??o da din?mica folicular em ?guas superovuladas com extrato de pituit?ria equina e FSH equino purificado. Disserta??o (Mestrado em Reprodu??o Animal) ? Universidade Estadual Paulista, Faculdade de Medicina Veterin?ria e Zootecnia, Botucatu, 2004. MARI, G., et al. Fertility in the mare after repeated transvaginal ultrasoundguided aspirations. Animal Reproduction Science, p.299-308, 2005. McCRACKEN, J.A, et al. Luteolysis: a neuroendocrine mediated event. Physiological Reviews, p.263-304, 1999. McKINNON, A.O., et al. Oocyte transfer in the mare: preliminary observations. Equine Veterinary Science, p.306-309, 1986. McKINNON, A.O & CARNEVALE E.M. Ultrassonography. In : Mckinnon, A.O. Equine Reproduction, p.211-220. 1993. McKINNON, A.O., et al. Ultrassonography. In : Mckinnon, A.O. Equine Reproduction, 2a Ed., 2011. MEYERS-BROWN, G.A., et al. Induction of ovulation in seasonally anestrous mares under ambient lights using recombinant equine FSH (reFSH). Theriogenology, p.456-462, 2013. MONTECHIESI, D.F. Efeito da aspira??o folicular sobre a concentra??o de progesterona plasm?tica em ?guas c?clicas. 2009, 78p. Disserta??o (Mestrado) Universidade Estadual Paulista ?J?lio De Mesquita Filho? Faculdade de Medicina Veterinaria e Zootecnia. Botucatu- SP, 2009. 28 MOON, Y.S, et al. Stimulation action of follicle stimulating hormone secretion by hypophysectomized rat ovaries in organ culture. Endocrinology, p. 244-247, 1975. MOZZAQUATRO, F.D., et al. Luteal function induced by transvaginal ultrasonic-guided follicular aspiration in mares. Animal Reproduction Science, p. 56?62, 2010. MOZZAQUATRO, F.D., et al. Progesterone production in mares and echographic evaluation of the corpora lutea formed after follicular aspiration. Reproduction Domestic Animals, p. 288?292, 2012. NAGY, P., et al. Seasonality in mares. Animal Reproduction Science, p. 245-262, 2000. Newcombe JR., et al. Treatment of transition phase mares with progesterone intravaginally and with deslorelin or Hcg to assist ovulations. Journal of Equine Veterinary Science, p.57-64, 2002. NIEKERK CH, et al. Progesterone treatment of mares with abnormal oestrous cycles early in the breeding season. Journal of the South African Veterinary Association, 1973. NISWENDER, G.D. Molecular control of luteal secretion of progesterone. Reproduction, p.333-339, 2002. NISWENDER & NETT. Luteal phase. In: VOSS, J.L.; McKINNON, A.O. (Eds.). Equine reproduction. Philadelphia: Lea & Febiger. p.172 175, 1993. NISWENDER, G.D, et al. Mechanisms controlling the function and life span of the corpus luteum. Hystology Reviews, p.1?29, 2000. NISWENDER, K.D., et al. Effect of purified equine folliclestimulating hormone on follicular development and ovulation in transitional mares. Equine Veterinary Science, p.37-9, 2004. 29 NODEN, P.A., et al. Early Changes in Serum Progesterone, Estradiol and LH during Prostaglandin F2?-induced Luteolysis in Mares. Journal of Animal Science, p. 666-671, 1978. PIERSON & GINTHER. Ultrasonic evaluation of the corpus luteum of the mare. Theriogenology, p.795-806, 1985. PIERSON, R. A. Folliculogenesis and ovulation. In: McKINNON, A. O., VOSS,J..L. Equine Reproduction. Philadelphia: Lea & Febiger. Cap. 17, p. 161-71, 1993. PIETERSE, M.C., et al. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology, p.751-762, 1988. RANDEL,R.D., el al. Exogenous PGF2? enhanced GnRH-induced LH release in postpartum cows. Theriogenology, 1996. RAZ, T., et al. Comparison of the effects of eFSH and deslorelin treatment regimes on ovarian stimulation and embryo production of donor mares in early vernal transition. Theriogenology, p. 358?1366, 2009. ROSER, J.F., et al. Luteal luteinizing hormone receptors during the postovulatory period in the mare. Biology of Reproduction, p.499-510, 1983. SALLES, M.G.F., et al. Corpo l?teo c?clico e gestacional: revis?o. Revista Brasileira de Reprodu??o Animal, n.3, p.185-194, 2010. SAMPER, J. C., et al. Current therapy in equine reproduction. Ed. Saunders Elsevier, 2? edi??o, St. Louis, Missouri, 492 p., 2007. SCHUTZER, C.G.C. Utiliza??o do implante de progesterona intra-vaginal e acetato de deslorelina em ?guas ac?clicas associados ou n?o a luz artificial para o controle da sazonalidade reprodutiva. 2012, 75p. Disserta??o (Mestrado). Universidade Estadual Paulista, Faculdade de Medicina Veterin?ria e Zootecnia, Botucatu- SP, 2012. 30 SCHUTZER, C.G.C., et al. Utiliza??o de diferentes per?odos de fotoestimula??o em ?guas ac?clicas para o controle da sazonalidade reprodutiva. Veterin?ria e Zootecnia, p.148-153, 2014. SENGER, P.L. Pathways to pregnancy and parturition. 2.ed. Washington: Current Conceptions Inc., 368p, 2003. Souza, R.T.R. et al. Sincroniza??o de receptoras no diestro para utiliza??o em programa de transfer?ncia de embri?es em equinos. Veterin?ria e Zootecnia, p. 245-253, 2015. SPICER, L.J., et al. Follicular fluid concentrations of free insulin-like growth factor (IGF)-I during follicular development in mares. Domestic Animal Endocrinology, p. 573 ? 581, 2005. SQUIRES, E.L., et al. Relationship of altrenogest to ovarian activity, hormone concentrations and fertility of mares. Journal Animal Science, p.901-910, 1983. STAEMPFLI, S.A., et al. Effect of a single injection of long-acting progesterone on the first ovulation in early and late spring transitional mares. Journal of Equine Veterinary Science, p. 744-748, 2011. STOCCO, C., et al. The molecular control of corpus luteum formation, function, and regression. Endocrine Reviews, p.117-149, 2007. STORER et al. Evaluation of injectable sustained release progestin formulations for suppression of estrus and ovulation in mares. Journal Reproduction Science, 2009. VIANA & BOLS. Vari?veis biol?gicas associadas a recupera??o de complexos cumulus-o?cito por aspira??o folicular. Acta Scientiae Veterinariae, p.1-2, 2005. WATSON, E.D., et al. Control of follicular development and luteal function in the mare: effects of a GnRH antagonist. Theriogenology, p.599-609, 2000. |
Page generated in 0.0028 seconds