Vitamin E sufficiency has been an on-going concern in premature infants particularly in ameliorating retinal artery abnormalities and in preventing hemolytic anemia. Prenatal vitamin supplements contain vitamin E, but the majority of these contain all-rat-a-tocopheryl acetate at l S mg or less. Using deuterium-labeled isotopes of RRR- and all-rac'-a-tocopheryl acetate, the transport of tocopherol during pregnancy has been investigated. Fifteen pregnant subjects were recruited 5 days prior to delivery to receive 15, 30. 75, 150 or 3 00 mg daily of a 1:1 mixture of d3-RRR- and d6-all-rat-a-tocopheryl acetate. Maternal blood samples were obtained throughout the dosing period along with cord blood samples, at parturition. Plasma, red blood cell and lipoproteins were evaluated by GC/MS to distinguish between JO, d3 and c/6 tocopherols Relative bioavailability of d6 was greater in maternal plasma at delivery when compared to Je at all dose levels. Cord blood samples exhibited greater cK tocopherol when compared to dt>. At the lowest dose levels 15,30 and 75 mg, cti/Jb in cord blood was 3 42 ±1.96 and 2 26 ±0.44 for the higher doses, respectively. Evaluation of lipoprotein tocopherol content revealed that LDL and HDL are the main transport lipoproteins in maternal blood with HDL being the main carrier in cord blood. We conclude that RRRa-tocopheryl acetate is more bioavailable than the all-rac- form and at low dose levels is the form transported preferentially by the placenta.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-14751 |
| Date | 01 December 1996 |
| Creators | Acuff, Robert V., Dunworth, R., Webb, L. |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | ETSU Faculty Works |
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