The Saccharomyces cerevisiae SKN7 gene has been identified through a search for genes which, at a high copy number, could restore the growth of the $kre9 Delta$ disrupted strain showing a cell wall $ beta$-glucan defect. SKN7 was mapped to the right arm of chromosome VIII, and is predicted to encode a 70 kDa protein, Skn7p, with a region of homology to the DNA binding domain of the yeast heat shock transcription factor, Hsf1p. Skn7p also has a domain which shows similarity to the prokaryotic receiver modules found on an extensive family of two-component response regulators, including the product of the rcsC gene. While restoring the growth rate to near wild type levels, SKN7 does not appear to restore the $ beta$-glucan levels of the $kre9 Delta$ mutant. However, SKN7-suppressed cells show a partially restored cellular morphology, and a restored cell wall resistance to mechanical stress. SKN7 does not suppress other mutations in the ($1 rightarrow6$)-$ beta$-glucan biosynthetic pathway, suggesting that it does not act as a general bypass suppressor of this glucan.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68231 |
| Date | January 1993 |
| Creators | North, Stan |
| Contributors | Bussey, A. Howard (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001396078, proquestno: AAIMM94489, Theses scanned by UMI/ProQuest. |
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