This thesis is a genetic study of the cytoplasmically- inherited determinant [psi] of Saccharomyces cerevisiae . [psi] is a potentiator of ochre suppression. The molecular basis of [psi] was investigated using mutagenesis as a probe. The psi<sup>+</sup> phenotype (efficient suppression) can be mutated to psi<sup>-</sup> phenotype (loss of suppression) by ultra-violet light (UV) and nitrosoguanidine (NTG) . The UV-induced mutation was a single-hit event and the pre-mutational lesion was partly photoreactivable . Repair or expression of UV-induced mutation to the [psi] determinant was under the same genetic control as for nuclear mutation. It was concluded that [psi] has a DNA genome. The 'extrachromosomal mutagens' thymidylate starvation, 5-fluorouracil, manganese chloride and cycloheximide failed to induce psi<sup>-</sup> mutants whilst guanidine hydrochloride, dimethyl sulphoxide and potassium chloride were shown to induce this mutation at frequencies up to 100%. Several other physical and chemical agents caused a high frequency of loss of the psi<sup>+</sup> phenotype. A new class of recessive nuclear mutation (pnm) was shown to cause a loss of the psi<sup>+</sup> phenotype. A simple comple- mentation test was devised to distinguish them from cytoplasmic psi<sup>-</sup> mutants. The dominant PNM<sup>-</sup> mutation was shown not to cause a physical loss of the [psi] genome. Two mutants with a modified PNM<sup>-</sup> phenotype were analysed. Attempts to demon- strate genetically the involvement of [psi] with the 80S ribosome were unsuccessful. The psi<sup>+</sup> phenotype was conclusively demonstrated to be inherited independently' of the nucleus using a 'heterokaryon test'. Two models for the [psi] phenomena were proposed; one postulating the presence of a DMA 'plasmid' and one postulating the involvement of a stable, self-perpetuating metabolic state.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:475637 |
| Date | January 1978 |
| Creators | Tuite, M. F. |
| Contributors | Cox, Brian G. |
| Publisher | University of Oxford |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://ora.ox.ac.uk/objects/uuid:5fbf8bef-22ce-4471-8d4e-b37f285343c1 |
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