We have previously demonstrated that Mid2p is required for the activation of the PKC1-MPK1 cell integrity pathway during cell exposure to mating pheromone, calcofluor white (CFW), and heat. Accumulating evidence indicates that Mid2p might regulate this pathway via the small GTPase, Rho1p. To understand the mechanism by which Mid2p signals, we initiated a two hybrid screen using the essential cytoplasmic tail of Mid2p as bait. ZEO1 (YOL109w), a previously uncharacterized open reading frame, was identified. ZEO1 encodes a 12kDa protein that co-localizes to the plasma membrane and interacts with the cytoplasmic tail of Mtl1p, a Mid2p functional homologue. Like mid2Delta mutants, cells deleted for ZEO1 are resistant to calcofluor white. In addition, ZEO1 null strains are no longer hypersensitive to calcofluor white caused by high copy expression of MID2. A role for Zeo1p in the cell integrity pathway is supported by the finding that disruption of ZEO1 leads to a Mid2p-dependent constitutive phosphorylation of Mpk1p. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33765 |
| Date | January 2002 |
| Creators | Green, Robin G. |
| Contributors | Bussey, Howard (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001871943, proquestno: MQ78882, Theses scanned by UMI/ProQuest. |
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