Aryl hydrocarbon hydroxylase (AHH) was induced 15-fold in Ambystoma tigrinum by intraperitoneal injection of 3-methylcholanthrene in corn oil, or 10-fold by addition of aromatic polycyclic hydrocarbons to the aqueous environment of the neotene animal. The cytochrome P-450-associated microsomal enzyme is similar to the inducible, one-gene, autosomal-dominant system typical in the laboratory mouse and man. Differences in optimal temperature for enzyme induction and activity were noted in organ culture of human and Ambystoma tissues, and ratios of benzpyrene metabolites differed between Ambystoma and Mus. The half life of enzyme activity induced in vivo was related to the excretion of hydrocarbon metabolites.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc663117 |
| Date | 12 1900 |
| Creators | Colvin, David P. |
| Contributors | Busbee, David L., Zimmerman, Earl G. |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | v, 57 leaves: ill., Text |
| Rights | Public, Colvin, David P., Copyright, Copyright is held by the author, unless otherwise noted. All rights |
Page generated in 0.002 seconds