Frozen section diagnosis (FSD) is a method implemented within the field of clinical histopathology and is used primarily intraoperatively to guide the surgical team, with regard to, for example, the severity and spread of the affliction. However, medical professionals typically refrain from relying on FSD due to the poor morphological outcome when compared to paraffin sectioning. There are several contributing factors for the lesser quality of FSD sections with one being the type of tissue evaluated and a prominent example of this is lung tissue.Optimizing the quality of sections obtained from FSD could provide more accurate diagnoses for patients.This thesis aims to assess various parameters related to FSD procedures to improve the morphological outcome. Comparisons between no injection and the injection of different solutions pre-freezing were made. Additionally, two different methods that are referred to as freehand- and tape-sectioning were compared. The results of the injections indicate that using O.C.T improves both the architecture of the tissue as well as the morphology. For practical reasons, O.C.T diluted 1:1 with saline was determined to be the best alternative to use in a clinical laboratory. When comparing the different sectioning methods, the Cryo-Jane tape transfer system produced a better section quality in the cases with injected O.C.T. Freehand sectioning did produce better results regarding connective tissue as well as cytoplasm. This means that depending on what parameters are deemed most important by the pathologist both methods have their distinct advantages.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-528723 |
| Date | January 2024 |
| Creators | Torstensson Bull, Frida |
| Publisher | Uppsala universitet, Institutionen för medicinsk cellbiologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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