In the urchin Strongylocentrotus purpuratus, the expression of the Spec3 gene is associated with the growth of cilia, one of the first morphogenetic events during development. The product of this gene was characterized using an antiserum raised against a peptide corresponding to the predicted amino-terminal portion of the protein. This thesis describes the localization of the Spec3 protein at different stages during embryonic development. Immunocytochemistry indicated that the protein is associated with cilia and Golgi complexes of ectodermal cells. Agents that inhibit protein synthesis and Golgi secretion also altered its normal distribution. Fractionation of cilia and immunoblotting indicate that the protein is associated with the ciliary axoneme and that it behaves as a large aggregate.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59519 |
| Date | January 1989 |
| Creators | Montpetit, Isabelle |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001067923, proquestno: AAIMM63645, Theses scanned by UMI/ProQuest. |
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