Diploma thesis deals with determination of vitamin C in various cultivars of sea buckthorn (Hippophae rhamnoides, L.) and quince (Cydonia oblonga, Mill.). In theoretical part, there are mentioned basic information about these less known fruit species, their medically significant substances and utilization of these fruits. Various methods for determination of vitamin C are discussed with focus on HPLC and extraction of vitamin C from the matrix. In experimental part, the most suitable column (Gemini C18, 150 x 4,6 mm; 5 m) was chosen from six various columns. Analysis of vitamin C in various cultivars of quince and sea buckthorn fruits were carried out on this column. The various types of matrix preparation and two different separation systems (method with oxalic acid and system with phosphate buffer and methanol as a mobile phases) were investigated too. Vitamin C was determined as an ascorbic acid.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216459 |
| Date | January 2009 |
| Creators | Cetkovská, Jitka |
| Contributors | Vránová, Dana, Vespalcová, Milena |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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