Regulation of tubulin gene expression in embryos of the sea urchin Lytechinus pictus has been experimentally investigated by use of cloned recombinant tubulin DNA and anti-tubulin antiserum. Tubulin synthesis appears to be autogenously regulated at the level of tubulin mRNA stability by the level of unpolymerized tubulin; i.e., the more unpolymerized tubulin, the less stable the tubulin mRNA. Destabilization of tubulin mRNA requires continued protein synthesis. Most of tubulin stored in eggs is unpolymerized; during embryogenesis the mass of tubulin per embryo changes little, but unpolymerized tubulin is increasingly polymerized into microtubules. There is a transcriptional stimulation of tubulin genes at the time of ciliogenesis but thereafter autoregulation by the ontogenetic decrease of the level of unpolymerized tubulin plays a predominant role for an increasing accumulation of tubulin mRNA. Deciliation results in a transient enhancement of transcription of tubulin genes, which is independent of the level of unpolymerized tubulin and does not require protein synthesis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74267 |
| Date | January 1987 |
| Creators | Gong, Zhiyuan. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000550045, proquestno: AAINN63436, Theses scanned by UMI/ProQuest. |
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