nÃo hà / O gÃnero Aniba destaca-se por suas propriedades farmacolÃgicas, tais como ansiolÃtica, antidepressiva e vasorelaxante. Dos frutos de Aniba panurensis conhecida popularmente por âlouro amareloâ foi identificada uma pirona natural, a 6-[(E)-esteril-piran-2-ona]. O objetivo do presente trabalho foi verificar os efeitos neurofarmacolÃgicos da 6-[(E)-esteril-piran-2-ona (STY) obtida da Aniba parusinensi em camundongos atravÃs de testes comportamentais (atividade locomotora espontÃnea (ALE), rearing e grooming), testes de induÃÃo de convulsÃo quÃmica e dosagens neuroquÃmicas de aminoÃcidos (glutamato (GLU), aspartato (ASP), Ãcido γ-aminobutÃrico (GABA), glicina (GLI), taurina (TAU), histidina (HIS) em cÃrtex prÃ-frontal (CPF), hipocampo (HC) e corpo estriado (CE). Foram utilizados camundongos Swiss, machos, com peso mÃdio de 28 gramas. Os animais foram tratados com dose Ãnica de STY (1, 5, 10 ou 20 mg) por via intraperitoneal. Trinta minutos apÃs administraÃÃo os animais foram submetidos aos testes comportamentais e teste de convulsÃo induzidas por pentilenotetrazol (PTZ), estricnina, bicuculina e pilocarpina. Imediatamente apÃs os testes os animais foram sacrificados e as Ãreas cerebrais de interesse dissecadas para a dosagem da concentraÃÃo de aminoÃcidos atravÃs de HPLC (High Perfomance Liquid Chromatography). No teste de ALE a STY na dose de 10 ou 20 mg/kg aumentou atividade locomotora quando comparada ao grupo controle. No Labirinto em cruz elevada e o teste da placa perfurada, a STY em todas as doses comprovou seu efeito ansiolÃtico, pois aumentou todos os parÃmetros analisados. Na dosagem de aminoÃcidos neurotransmissores apÃs o teste de comportamento houve aumento nas concentraÃÃes dos aminoÃcidos inibitÃrios (GABA, GLI, TAU, HIS) e excitatÃrios (ASP, GLU) no CPF, HC e CE. ApÃs o prÃ-tratamento com STY os animais foram submetidos ao teste de induÃÃo de convulsÃo por PTZ (85 mg/kg) ou Bicuculina (12 mg/kg) foram observados aumentos na latÃncia de convulsÃo e morte, com animais sobreviventes na maior dose. No teste com a induÃÃo de convulsÃo por estricnina (20 mg/kg/ ocorreu o aumento na latÃncia de convulsÃo (STY-10: 50,42  7,20; STY-20: 65,99  3,22) e latÃncia de morte (STY-1: 20  1,72; STY-10: 19,17  1,87; STY-20: 23,83  1,55) em todos os animais prÃ-tratados com STY. Na induÃÃo de convulsÃo por pilocarpina ocorreu uma diminuiÃÃo da latÃncia de morte. ApÃs os testes os animais realizou-se a dosagem da concentraÃÃo dos aminoÃcidos (ASP, GLU, GLI, TAU e GABA). No CPF aumentaram ASP, TAU, GABA. Jà no HC o aumentou GLU, ASP, GLI e GABA. Na induÃÃo de convulsÃo com estricnina no CPF ocorreu o aumento no ASP, TAU, GABA, enquanto no HC aumentou ASP, GLI, TAU, GABA. No modelo de induÃÃo com bicuculina no CPF aumentou o ASP, GLU, GLI, TAU, GABA, enquanto no HC aumentou ASP, GLU, GLI, TAU, GABA. No modelo de induÃÃo com pilocarpina no CPF aumentou ASP, GABA, GLI, enquanto no HC aumentou apenas o ASP. Conclui-se que a STY apresenta um efeito ansiolÃtico nos testes de comportamento, efeito protetor nos teste de induÃÃo de convulsÃo por PTZ, estricnina e bicuculina. ApÃs o doseamento dos aminoÃcidos pode-se demonstrar a participaÃÃo dos sistemas glutamatÃrgico, GABAÃrgico e glinÃrgico. / The genus Aniba stands out for its pharmacological properties, such as anxiolytic, antidepressant and vasorelaxant. Of the Aniba panurensis fruits popularly known as ―louro amarelo‖ a natural pyrone, 6-[(E)-styryl-pyran-2-one], was identified. The objective of this study was to verify the neuropharmacological effects of 6-[(E)-styryl-pyran-2-one] (STY), obtained from Aniba panurensis, in mice through behavioral tests (spontaneous locomotor activity (SLA), rearing and grooming), tests of chemically induced seizures and neurochemical dosages of amino acids (glutamate (GLU), aspartate (ASP), γ-aminobutyric acid (GABA), glycine (GLY), taurine (Tau), histidine (HIS) in the prefrontal cortex (PFC), hippocampus (HC) and striatum (ST). We used Swiss mice, male, with an average weight of 28 grams. The animals were treated with a single dose of STY (1, 5, 10 or 20 mg) by intraperitoneal injection. Thirty minutes after administration, the animals were subjected to behavioral tests of chemically induced seizures by pentylenetetrazol (PTZ), strychnine, bicuculline and pilocarpine. Immediately after the tests, the animals were sacrificed and the brain areas of interest were dissected to estimate the amino acid concentration via HPLC (High Performance Liquid Chromatography). In the SLA test the STY at 10 or 20 mg/kg dose increased locomotor activity when compared to the control group. In the elevated plus-maze and hole-board tests, the STY in all doses proved its anxiolytic effect, because it increased all parameters analyzed. In the dosage of amino acid neurotransmitters after behavioral test there was an increase in inhibitory amino acid concentrations (GABA, GLY, TAU, HIS) and excitatory (ASP, GLU) in PFC, HC and ST. After pretreatment with STY, the animals were tested for seizures induced by PTZ (85 mg/kg) or Bicuculline (12 mg/kg), we observed an increase in the latency of seizures and death in surviving animals at the highest dose. During the strychnine-induced seizures test (20 mg/kg) there was an increase in seizure latency (STY-10: 50.42  7.20; STY-20: 65.99  3.22) and latency to death (STY-1: 20  1.72; STY-10: 19.17  1.87; STY-20: 23.83  1.55) in all animals pretreated with STY. In the pilocarpine-induced seizures there was a decrease in the latency to death. After testing the animals, we conducted the dosage of amino acid concentrations (ASP, GLU, GLY, TAU and GABA). PFC increased ASP, TAU and GABA. HC increased GLU, ASP, GLY and GABA. In the strychnine-induced seizure in PFC there was an increase in ASP, TAU and GABA, while the HC increased ASP, GLY, TAU and GABA. In the bicuculline-induced seizure in PFC there was an increase in ASP, GLU, GLY, TAU and GABA, while the HC increased ASP, GLU, GLY, TAU and GABA. In the pilocarpine-induced seizure in PFC there was an increase in ASP, GABA, GLY, while the HC increased only ASP. We concluded that STY presents an anxiolytic effect in behavioral tests and a protective effect in tests of induced seizures by PTZ, strychnine and bicuculline. After the dosage of the amino acids we can demonstrate the involvement of glutamatergic, GABAergic and glycinergic systems.
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:6189 |
| Date | 25 June 2012 |
| Creators | Edna Maria Camelo Chaves |
| Contributors | SilvÃnia Maria Mendes Vasconcelos, Francisca ClÃa FlorenÃo de Sousa, Lissiana Magna Vasconcelos Aguiar, Luzia Kalyne Almeida Moreira Leal, Paula Matias Soares |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Farmacologia, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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