Abstract
RF-gradients produced utilizing RF-field inhomogenity of conventional receiver/transmitter coil of NMR-probe can be used to mimic the effects of B0-gradients. This is done by placing long inhomogenous pulse in between two 90° pulses of appropriate phases (z-rotation cluster). B0-gradient based excitation sculpting can be converted into RF-gradient version. Selective one-dimensional TOCSY and NOESY using RF-gradient based excitation sculpting are described. In addition, non-selective two-dimensional experiments, TOCSY and NOESY, with RF-gradient based coherence selection are presented. Excitation sculpting using BIRD or BIRDR as inversion element results in isotope filter. Pre-suppression of non-13C-bound protons using RF-gradient BIRD prior to HSQC enables recording of spectrum of comparable quality to B0-gradient selected HSQC. This is beneficial for spectrometers lacking B0-gradient capabilities. Excitation sculpting using BIRDR can be used efficiently as low-pass filter in HMBC experiment. Furthermore, simultaneous elimination of protons bound to 15N and 13C can be accomplished with BIRDR based method.
| Identifer | oai:union.ndltd.org:oulo.fi/oai:oulu.fi:isbn951-42-5235-7 |
| Date | 11 May 1999 |
| Creators | Heikkinen, S. (Sami) |
| Publisher | University of Oulu |
| Source Sets | University of Oulu |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis, info:eu-repo/semantics/publishedVersion |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess, © University of Oulu, 1999 |
| Relation | info:eu-repo/semantics/altIdentifier/pissn/0355-3191, info:eu-repo/semantics/altIdentifier/eissn/1796-220X |
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