The aim of this thesis was to evaluate the effect of increased intake of selenium from the diet of rats with spontaneous hypertension (SHR) on other essential elements. The effect of different diets on the element levels in kidney, liver, testes, and blood of rats was observed under model conditions. Tested rats were divided into five groups, where the first group A was the reference group and feed mixture contained no additive. In groups B and C was soybean meal replaced by defatted rapeseed and into feed mixture of group C was additionally added vitamin E. Diet groups D and E contained added selenium and zinc, and group E contained additionally vitamin E in comparison to reference group A. Se content in kidney, liver, testicles, and blood of animals was determined by inductively coupled plasma mass spectrometry (ICP MS). The other essential elements (Cu, Fe, Mn, P, S, and Zn) were determined by inductively coupled plasma optical emission spectrometry (ICP OES). The content of Ca, K and Mg was determined by atomic absorption spectrometry with flame atomizer (F-AAS). The hypothesis has been that the increased intake of selenium should improve the use of other essential elements by the rat organism. It was expected that there will be influenced elements mainly involved in the defense of the organism against oxidative stress. This was not confirmed by this experiment. Changes in the concentrations of these elements occurred only at very high dose of selenium that is already toxic to the organism. This toxic effect could be also responsible for fifty percent mortality of rats in group E, where perhaps even increased concentration of vitamin E could not sufficiently reduce the toxicity of high doses of selenium.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:315904 |
| Date | January 2017 |
| Creators | Růnová, Karolína |
| Contributors | Száková, Jiřina, Václav, Václav |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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