Alkylphenol polyethoxylates (APEOs) are commonly present in both industrial and municipal wastewaters. They belong to the nonionic surfactants which have been widely used for years. APEOs themselves are nontoxic to organisms. When released into the environment, the EO chain of APEOs are degraded chemically and biologically. Some of the products, such as nonylphenol and octylphenol, are persistant. According to the partition coefficient constant, the alkylphenols are adsorpted in the sediments and accumulated in the environment. Nonylphenol (NP) is an analog of 17£]-estradiol, a sex hormone. It is one of the environmental hormones which can get into the organisms through the food chain and may interfere with the reproduction function. Previous studies showed the Love River in Kaohsiung was polluted with APEOs considerably. Bacteria capable of using nonylphenol as the sole carbon source were isolated by the enrichment procedures. Some of the bacterial isolates were identified as Serratia marcescens (strain A), Vibrio sp. (strain B) and Aeromonadaceae sp. (strain C) by the 16S rRNA phylogeny. The rates of NP degradation were evaluated by the HPLC-UV. S. marcescens strain A manifested the best degradative. It could degrade almost 25 ppm of NP in 28 days. The degradative capability of Vibrio sp. (strain B), Aeromonadaceae sp. (strain C) and the enriched mix culture were 65%, 25% and 30%, respectively. Additionally, to know the limitation of degrading nonylphenol by strain A, we set the concentration to 100 ppm for the test. Strain A could degrade 72 ppm in this test. Bacterial composition of the enriched consortia was grouped by the DGGE method. The dominants were Ochrobactrum sp. and Alcaligenaceae sp. which may be to applied to environmental bioremediation.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0821109-152509 |
| Date | 21 August 2009 |
| Creators | Huang, Wei-chun |
| Contributors | Zhi-Hong Wen, Chi-Hsin Hsu, Chan-Shing Lin |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0821109-152509 |
| Rights | not_available, Copyright information available at source archive |
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