SRY (Sex determining Region of Y chromosome) is the master gene initiating testis determination in mammals. To shed light on the molecular mechanism of SRY action during testicular differentiation, we examined the effects of TAT-HMG fusion protein on gonadal sex differentiation in culture. HMG is the DNA binding motif of SRY and "TAT" is a protein transduction domain. Each pair of CD1 mouse gonadal primordia at 11.5 days post coitum (dpc) was cultured with or without TAT-HMG dissolved in dimethyl sulfoxide (DMSO) up to 3 days. Immunocytochemical labeling and Real-time RT-PCR of Sry, Sox9 and Mis indicated that DMSO blocked testicular differentiation, Sertoli cell differentiation and testis cords formation, downstream of SRY. TUNEL showed a massive mesenchymal cell death, which might be responsible for disruption of testis cord formation. Treatment with TAT-HMG rescued Sertoli cell differentiation, probably by up regulation of Sry, but not testis cord formation or cell death.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.112313 |
| Date | January 2007 |
| Creators | Tavallaee, Ghazaleh. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002711491, proquestno: AAIMR51350, Theses scanned by UMI/ProQuest. |
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