by Xie Weijun. / Publication date from spine. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 107-122). / ABSTRACT --- p.i / ACKNOWLEDGMENTS --- p.iii / TABLE OF CONTENTS --- p.iv / LIST OF FIGURES --- p.vii / LIST OF TABLES --- p.x / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 2. --- LITERATURE REVIEW --- p.4 / Chapter 2.1 --- Economic and biotechnological significance of Lentinula edodes --- p.4 / Chapter 2.2 --- Biological background --- p.8 / Chapter 2.2.1 --- Life cycle --- p.8 / Chapter 2.2.2 --- Morphological changes during fruit body development --- p.9 / Chapter 2.2.3 --- Biochemistry of fruit body development --- p.13 / Chapter 2.2.4 --- Molecular studies --- p.17 / Chapter 2.3 --- RNA AP-PCR --- p.23 / Chapter 3. --- MATERIALS AND METHODS --- p.26 / Chapter 3.1 --- Biological materials --- p.26 / Chapter 3.2 --- Media --- p.26 / Chapter 3.3 --- RNA AP-PCR with RNAs isolated from four different developmental stages --- p.27 / Chapter 3.3.1 --- Isolation of total RNAs --- p.27 / Chapter 3.3.2 --- RNA AP-PCR --- p.30 / Chapter 3.3.3 --- PCR reamplification --- p.33 / Chapter 3.4 --- PCR cloning and screening --- p.33 / Chapter 3.4.1 --- PCR product purification --- p.34 / Chapter 3.4.2 --- Cloning --- p.34 / Chapter 3.4.3 --- Transformation --- p.36 / Chapter 3.4.4 --- Screening --- p.36 / Chapter 3.4.4.1 --- Restriction enzyme digestion of plasmid DNA --- p.36 / Chapter 3.4.4.2 --- PCR Screening --- p.38 / Chapter 3.5 --- DNA sequencing --- p.38 / Chapter 3.5.1 --- Preparation of double-stranded template --- p.38 / Chapter 3.5.2 --- Sequencing reaction using double-stranded templates --- p.39 / Chapter 3.5.3 --- Electrophoresis --- p.40 / Chapter 3.5.4 --- Autoradiography and analysis --- p.41 / Chapter 3.6 --- Dot-blot analysis --- p.41 / Chapter 3.6.1 --- Dot-blot --- p.41 / Chapter 3.6.2 --- Probe preparation --- p.42 / Chapter 3.6.3 --- Prehybridization and hybridization --- p.43 / Chapter 3.6.4 --- Autoradiography and Analysis --- p.43 / Chapter 3.7 --- Study on one clone: the gene encoding ubiquitin --- p.44 / Chapter 3.7.1 --- "Design of primers:UBR5A, UBR3A and Polyub5A, polyub3A" --- p.44 / Chapter 3.7.2 --- Isolation of DNA from mycelium and fruit body --- p.46 / Chapter 3.7.3 --- Specific PCR with Polyub primers --- p.46 / Chapter 3.7.4 --- RT-PCR --- p.47 / Chapter 4. --- RESULTS --- p.48 / Chapter 4.1 --- Total RNA isolation --- p.48 / Chapter 4.2 --- RNA AP-PCR --- p.52 / Chapter 4.3 --- PCR reamplification --- p.58 / Chapter 4.4 --- Molecular cloning of RAP products --- p.58 / Chapter 4.5 --- Screening --- p.61 / Chapter 4.6 --- DNA sequencing --- p.65 / Chapter 4.7 --- Sequence analyses --- p.80 / Chapter 4.8 --- Dot blot --- p.84 / Chapter 4.9 --- Further study on pMrG290a --- p.84 / Chapter 5. --- DISCUSSION --- p.92 / Chapter 5.1 --- RNA AP-PCR --- p.92 / Chapter 5.2 --- PCR cloning --- p.94 / Chapter 5.3 --- Nucleotide sequencing --- p.95 / Chapter 5.4 --- Dot blot --- p.96 / Chapter 5.5 --- Effect of polyphenol in RNA or DNA samples to the efficiency of reverse transcription and PCR --- p.98 / Chapter 5.6 --- Ubiquitin and fruit body development --- p.99 / Chapter 5.7 --- "Mitochondrial biogenesis, bioenergetics and fruit body development" --- p.102 / CONCLUSION --- p.105 / REFERENCES --- p.107
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321618 |
| Date | January 1996 |
| Contributors | Xie, Weijun., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 122 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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