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Construction and Applications of Two-photon Micro-spectroscopy

In this thesis the effects of single photon and multi-photon excitation on protoplasts from Arabidopsis thaliana are compared. Time-lapsed micro-spectroscopy at high spatial resolution is employed to study the response of chloroplasts within the protoplasts from Arabidopsis thaliana. We have found that the fluorescence spectra of chloroplasts exhibits dramatic changes and the protoplasts are rapidly damaged under multi-photon excitation as a result of pulsed laser illumination. In contrast, single photon excitation of chloroplasts with cw laser is relatively inert to the vitality of the protoplasts. In addition to, we have built an ultrafast laser excited cryogenic micro-spectroscopy setup to study the photoluminescence of PPV thin film. We found that the spectrum of PPV¡¦s photoluminescence should shift toward longer wavelength and the non-radiative transition should be suppressed as a result of longer electron coherence length at low temperature.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0703101-150440
Date03 July 2001
CreatorsWang, Yi-Ming
ContributorsChu-Kuang Sun, Wood-Hi Cheng, Tuh-Jzeng Lue, Sheng-Lung Huang, Fu-Jen Kao
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0703101-150440
Rightsunrestricted, Copyright information available at source archive

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