Single-cell Sequencing Studies of Somatic Mutation in the Human Brain

Description

A major unanswered question in neuroscience is whether there exists genomic variability between individual neurons of the brain, contributing to functional diversity or to an unexplained burden of neurologic disease. To address this question, we developed methods to amplify genomes of single neurons from human brains, achieving >80% genome coverage of single-cells and allowing study of a wide-range of somatic mutation types.

Links & Downloads

1. Evrony, Gilad David. 2013. Single-cell Sequencing Studies of Somatic Mutation in the Human Brain. Doctoral dissertation, Harvard University.
2. http://dissertations.umi.com/gsas.harvard:10747
3. http://nrs.harvard.edu/urn-3:HUL.InstRepos:11151539

Tags

Biology

Neurosciences

Genetics

Brain

Single-cell sequencing

Somatic mutation

Transposons

Additional Fields

Identifer	oai:union.ndltd.org:harvard.edu/oai:dash.harvard.edu:1/11151539
Date	January 2013
Creators	Evrony, Gilad David
Contributors	Walsh, Christopher A
Publisher	Harvard University
Source Sets	Harvard University
Language	en_US
Detected Language	English
Type	Thesis or Dissertation
Rights	closed access