We have searched the bioactive metabolites from the wild-type and cultured soft corals Sinularia flexibilis. This study led to the isolation of fifteen natural products (1¡V15), including four new metabolites (1¡V4), flexibilisolide A (1), flexibilisin A (2), quercifomolide D (3) and quercifomolide B (4) along with eight known compounds (5¡V12) from the wild-type soft coral S. flexibilis, and three new metabolites (13¡V15), flexibilisolide B (13), flexibilisin B (14), flexibilisquinone (15) along with four known compounds (9¡V12) from the cultured soft coral S. flexibilis. The structures of metabolites 1¡V15, including their stereochemistries, have been established by detailed spectroscopic analyses and by comparison with the related physical and spectral data from other known compounds. The relative configuration of metabolite 4 was further confirmed by X-ray single-crystal diffraction analysis. Furthermore, the absolute configuration of 4 was determined by a modified Mosher¡¦s method.
The cytotoxicity of 1¡V15 against the growth of human tumor cell lines, including cervical epitheloid (Hela), laryngeal (Hep 2), medulloblastoma (Daoy) and breast (MCF-7) carcinoma cells was studied. The results showed that only compounds 7, 9 and 11 exhibited moderate cytotoxicities against the tested cell lines. Furthermore, compounds 1, 6, 8, 9, 11¡V13 and 15 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein in the LPS-stimulated RAW264.7 macrophage cells at 10 £gM. At the same concentrations, compounds 11 and 12 also could significantly inhibit the accumulation of the pro-inflammatory COX-2 protein.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0905108-175839 |
| Date | 05 September 2008 |
| Creators | Lin, Yu-fang |
| Contributors | Yang-Chang Wu, Jyh-Horng Sheu, Ping-Jyun Sung, Fag-Rong Chang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905108-175839 |
| Rights | not_available, Copyright information available at source archive |
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