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Studies on the Secondary Metabolites from the Soft Corals Sinularia leptoclados and Sinularia sandensis

Soft corals of the genus Sinularia have been proved to yield a wide variety of secondary metabolites. In order to search for novel bioactive substances from marine organisms, we have investigated the secondary metabolites of the soft corals Sinularia leptoclados and Sinularia sandensis. Chromatographic separation of the organic extracts of the
Formosan soft coral Sinularia leptoclados, collected at Dongsha Atoll of the South China Sea, led to the isolation of four new steroids (1-3 and 11), along with an unexpected artificial sterol (5) and six known 9,11-secosteroids (4 and 6-10). In addition, the acetone extract of the soft coral, Sinularia sandensis, collected at the Tsau-Lou-Cho Island off the southwestern coast of Taiwan, yielded three novel sesquiterpenoids (12-14). The structures of the isolated metabolites were elucidated by extensive spectroscopic analyses (IR, UV, mass, optical rotations, CD, 1D and 2D NMR) and by comparison of the spectral data with those of the related known compounds. Moreover, the absolute configuration of 12 was established by application of modified Mosher¡¦s method. The cytotoxicity against of P-388 (mouse lymphocytic leukemia), HT-29 (human colon adenocarcinoma), and A-549 (human lung epithelial carcinoma) cells of metabolites 1-4 and 6-14 as well as the anti-HCMV (human cytomegalovirus) activities of metabolites 4, 10, 11, and 13 were evaluated. Metabolites 2, 4 and 6-10 exhibited significant activity against P-388 cell in vitro (IC50¡Ø4 mg/mL).

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0823110-122257
Date23 August 2010
CreatorsChen, Pei-wen
ContributorsLing-ching Chen, Chang-yih Duh, Ian-lih Tsai
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823110-122257
Rightsnot_available, Copyright information available at source archive

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