RNA interference represents a useful tool for modulating expression of sarcosine metabolism genes and for studying the role of sarcosine in prostate cancer. The thesis "Modulation of sarcosine metabolism by RNA interference" summarizes current state-of-the-art of possible regulation of gene expression using various types of nucleic acids. Furthermore the thesis also deals with the issue of the transfer of these regulatory agents to target tissues and finally it describes sarcosine including its involvement in the metabolic cycles of the cell. The main aim of the experimental part of this work was to determine the influence of knock down sarcosine dehydrogenase (SARDH) on other enzymes involved in sarcosine metabolism. This effect was assessed by determining the gene expression of individual genes encoding the four major sarcosine pathway enzymes by quantitative real-time PCR analysis. Experiments were performed on three different prostatic cell line types, PNT1A, DU-145 and PC3. The most significant differences on level of gene expression were observed in carcinoma cells DU-145 in which a significant increase in gene expression of dimethylglycine dehydrogenase (DMGHD) and sarcosine oxidase (PIPOX) was observed after applications of siRNA targeting the SARDH.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:427877 |
| Date | January 2019 |
| Creators | Šubrtová, Hana |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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