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Previous issue date: 2018-03-28 / Prostate cancer (PC) is the second most diagnosed neoplasm in men, with the exception of non- melanocytic skin tumors. Its global incidence is 1.1 million cases per year and its mortality rate is around 307,000 deaths. Around the world, there are 3,850,000 CP survivors, since their survival in five years reaches values exceeding 80%. Therefore, adequate stratification in the diagnosis and follow-up of these patients is so relevant. In this study, we aimed of evaluating ATP / ADP / AMP hydrolysis as a prognostic biomarker in the CP; CD39 and CD73 enzymes are known to be responsible for the transformation of ATP or ADP into AMP and into adenosine, respectively. Blood samples were collected from 29 patients treated at the Cruz Alta Oncology and Hematology Center (COHCA) and 17 healthy controls (CS), in order to evaluate the hydrolysis activity of ATP, ADP and AMP and to correlate the data between the cases and controls. In addition, the results of hydrolysis were correlated with recognized prognostic factors as Gleason Score (EG), clinical stage (EC) and PSA levels. The mean age of the selected patients was 63.3 years, 71.4% presented PSA <10 at diagnosis, 82.7% were grouped as low in EG and 48.3% of patients belonged to EC IIB. The results demonstrated statistically significant differences in the nucleotide hydrolysis profiles of the cases and controls. In the CP patients the AMP hydrolysis was higher when compared to the hydrolysis of ATP and ADP. Regarding the clinical staging, it was observed that the hydrolysis of ATP was higher in the initial clinical stages, whereas the AMPase remained high regardless of clinical stage. Therefore, we can conclude that the understanding of the ectonucleotidases activities and their influence on tumor progression can collaborate for the diagnostic and improve the therapeutic approach for this neoplasia. / O c?ncer de pr?stata (CP) ? a segunda neoplasia mais diagnosticada no sexo masculino, excetuando-se os tumores de pele n?o melanoc?ticos, por ano sua incid?ncia mundial ? de 1,1 milh?es de casos e sua taxa de mortalidade de cerca de 307.000 ?bitos. Ao redor do mundo h? 3.850.000 sobreviventes de CP, visto que sua sobrevida em cinco 5 anos chega a valores superiores a 80%. Portanto, a estratifica??o adequada no diagn?stico e no seguimento destes pacientes ? muito relevante. Neste estudo objetivou-se avaliar a hidr?lise dos nucleot?deos ATP/ADP/AMP como biomarcadores progn?sticos no CP. Sabidamente as enzimas CD39 e CD73 s?o as principais respons?veis, respectivamente, pela transforma??o de ATP ou ADP em AMP, e este em adenosina. Foram coletadas amostras sangu?neas de 29 pacientes tratados no Centro de Oncologia e Hematologia de Cruz Alta ? COHCA e 17 controles sadios (CS), com o objetivo de avaliar a atividade de hidr?lise do ATP, ADP e AMP e correlacionar os dados entre os casos e controles. Al?m disso, os dados de hidr?lise foram correlacionados com fatores progn?sticos reconhecidos como escala de Gleason (EG), est?gio cl?nico (EC) e n?veis de PSA. A m?dia de idade dos pacientes selecionados foi de 63,3 anos, 71,4% apresentavam PSA < 10 no diagn?stico, 82,7% estavam agrupados como baixo grau na EG e 48,3% dos pacientes pertenciam ao EC IIB. Os resultados demonstraram diferen?as estatisticamente significativas nos perfis de hidr?lise de nucleot?deos entre casos e controles. Nos portadores de CP a hidr?lise do AMP foi mais elevada quando comparada com as hidr?lises de ATP e ADP. Em rela??o ao estadiamento cl?nico, observou-se que a hidr?lise de ATP foi maior nos est?gios cl?nicos iniciais, enquanto que a AMPase se manteve elevada independentemente do est?gio cl?nico. Portanto, pode-se concluir que o estudo da atividade das enzimas ectonucleotidases e o entendimento do CP pode colaborar na compreens?o dos fatores que influenciam a progress?o tumoral, visando melhorar o diagn?stico e a abordagem terap?utica para esta neoplasia.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/8163 |
| Date | 28 March 2018 |
| Creators | Gardani, Carla Fernanda Furtado |
| Contributors | Morrone, Fernanda Bueno |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, Brasil, Escola de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 500, 500, -224747486637135387, -969369452308786627 |
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