<p> Colonies of the soft coral Sinularia polydactyla collected from the vicinity of
Panjang island, Jepara region, Central Java, Indonesia were tested to examine their
antibacterial properties. </p> <p> Chemical analysis and bioassay-directed purification by repetitive column
chromatography using Vibrio harveyi as tester strain revealed that S. polydactyla
possesses antibacterial properties against this test strain. Purification gives a mixture of
esters, which inhibited the growth of the marine biofilm forming bacterium, V. harveyi
with an EC50 value of 0.075 mg/10 mL of culture medium. </p> <p> Chemical synthesis of the esters Hexadecyl palmitate (1 ), hexadecyl stearate (2),
octadecyl palmitate (3), and octadecyl stearate (4) was accomplished from the alcohols
and fatty acid acyl halides. Each purified ester was characterized by NMR and MS.
Comparison of the MS for these authentic synthetic samples with that for the biologically
derived sample showed that the latter was composed predominantly of esters 1 and 3.
The individual esters were tested against V. harveyi. Compound 1 was active and 4 was
less active, while 2 and 3 were inactive. Hence, 1 is the major active compound against
V. harveyi in the natural mixture. </p> / Thesis / Master of Science (MSc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/22445 |
| Date | 07 1900 |
| Creators | Radjasa, Ocky |
| Contributors | Risk, Michael, Harrison, Paul, Biology |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
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