Eighteen eunicellin-based diterpenoids were isolated from the organisms of the soft coral Cladiella krempfi. According to the results of 1D and 2D NMR spectroscopic experiments, the structures of these marine natural products were established. Among them, krempfielins A¡VJ (1¡V10) are new compounds, and sclerophytin B (11), sclerophytin F (12), litophynols A (13), (1R*, 2R*, 3R*, 6S*, 7S*, 9R*, 10R*, 14R*)-3-butanoyloxycladiell-11(17)-en-6,7-diol (14), litophynin I monoacetate (15), (1R*, 2R*, 3R*, 6S*, 9R*, 10R*, 14R*)-3- acetoxycladiell-7(16)-11(17)-dien-6-ol (16), 6-acetoxy litophynin E (17) and litophynin F (18) are the previously isolated compounds.
The anti-inflammation activity of these compounds at 10 £gM was studied. The results showed that 9, 14 and 17 effectively inhibited more than 80% expression of iNOS protein in LPS stimulated RAW 264.7 macrophage cells, and 5, 6, 10, 13 and 18 inhibited more than 60% expression of iNOS protein. Also, 9, 12, 16 and 18 were found to inhibit the expression of COX-2 protein in LPS stimulated RAW 264.7 macrophage cells to 52.5, 79.2, 82.2 and 48.2%. Cytotoxicity of these compounds were tested by the MTT assay using five human cancer cell lines (A549, BT483, H1299, Hep3B and SAS) and one normal cell line, BEAS2B. Compounds 9, 10, 11, 14, 17 and 18 were shown to exhibit cytotoxic activity.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0825110-173706 |
| Date | 25 August 2010 |
| Creators | Tai, Chi-jen |
| Contributors | Yang-Chang Wu, Jyh-Horng Sheu, Ping-Jyun Sung, Fang-Rong Chang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0825110-173706 |
| Rights | not_available, Copyright information available at source archive |
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