In order to discover for bioactive compounds, we have studied the chemical constituents from the organic extracts of soft coral Klyxum molle. This study had led to the isolation of 16 eunicellin-type diterpenoids, including ten new compounds, klymollins A¡VJ (1¡V10), along with six know compounds 11¡V16. The structures of compounds 1¡V16 were established by spectroscopic methods and by comparison of the spectral data with those of the related known compounds. It is noteworthy to mention that compounds 1¡V10 represent the first example of eunicellins possessing a C-11/C-17 epoxide. The absolute configuration of 4 was determined using a modified Mosher¡¦s method.
The cytotoxicity of compounds 1¡V8 and 11¡V16 against the A549 (human lung epithelial cells), HepG2 (human hepatocellular carcinoma), Hep3B (human hepatocellular carcinoma), MCF-7 (human breast adenocarcinoma)¡BMDA-MB231 (human breast adenocarcinoma) tumor cell lines were determined. Compound 11 showed cytotoxicity toward A549 tumor cells (IC50 value of 3.14 £gg/mL) and compounds 15 and 16 were found to exhibit cytotoxicity toward HepG2 tumor cell (IC50 values of 3.82 and 2.50 £gg/mL). Compounds 6 and 7 were found to show significant activity against the accumulation of the pro-inflammatory iNOS and COX-2 protein at 1 £gM.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0816110-202443 |
| Date | 16 August 2010 |
| Creators | Hsu, Fang-Jung |
| Contributors | Fang-Rong Chang, Ping-Jyun Sung, Jyh-Horng Sheu, Yang-Chang Wu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0816110-202443 |
| Rights | not_available, Copyright information available at source archive |
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