Soil samples were collected from several corn fields with history of atrazine (herbicide) application. Samples were inoculated into Erlenmeyer flasks each containing 50m1 of minimal basal salts medium amended with 100 ppm atrazine as sole nitrogen source. Flasks were shaken at 200 rpm at ambient temperature and were examined daily for one week for microbial growth and/or disappearance of atrazine. Promising consortia were subcultured for further additional enrichments before characterization of potentially active protein (enzyme)fractions. Proteins from cell-free and cellbound fractions were compared for ability to denature atrazine. Following gel permeation chromatography, isolated protein fractions were examined for atrazinefound in the cell-bound fractions capable degrading degradation. Two were found in the cell-free fractions (approx. Mol. wts. 55kDa and 180kDa) and one (55 kDa) atrazine to hydroxyatrazine. Atrazine and its breakdown products (hydroxyatrazine in particular) were detected via HPLC using C18 and C8 columns with absorbance at 229 nm. / Department of Biology
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/185872 |
| Date | January 1997 |
| Creators | Maleki, Saber Haghighati |
| Contributors | Ball State University. Dept. of Biology., Warnes, Carl E. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | v, 59 leaves : ill. ; 28 cm. |
| Source | Virtual Press |
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